96 Program for Research in Science and Engineering Assessing Protein Preservation in Historically Preserved Lung Tissue

Authors:

Juliet Garon, Ashley Scott, Christina Warinner

Date Created:

2025-01-01

Course Title:

Professor:

Not specified

About Paper:

Ancient and historical proteins are incredibly difficult to recovermost prevalent preserved protein signatures. The majority of and analyze, as tissue samples are potentially subject to handling,samples yielded identifiable protein signatures, largely consisting unknown storage conditions, and degradation. This study analyzes of neutrophil defensins, hemoglobin, and myoproteins. This study historical lung tissue from individuals suspected of tuberculosis is a precursor to a larger project searching for pathogen-specific andinfluenzainfection. Eachlungwaspreservedinformaldehyde- protein signatures in archeological samples, thereby allowing based solvent, adding to the difficulty of protein analysis due reconstructionofthetemporalandgeographicalspreadofdiseases. to formaldehyde’s crosslinking and denaturing effects. Protein As recovery from the majority of lung samples was relatively low extraction analysis was performed in a clean room and analyzed and not pathogen-specific, next steps will be to optimize extraction via liquid chromatography mass spectroscopy in order to identify protocols for pathogenic proteins in calcified samples. InvestigatingtheRoleofSpiralGanglionNeuronDegenerationinaTMPRSS3

Abstract:

Ancient and historical proteins are incredibly difficult to recovermost prevalent preserved protein signatures. The majority of and analyze, as tissue samples are potentially subject to handling,samples yielded identifiable protein signatures, largely consisting unknown storage conditions, and degradation. This study analyzes of neutrophil defensins, hemoglobin, and myoproteins. This study historical lung tissue from individuals suspected of tuberculosis is a precursor to a larger project searching for pathogen-specific andinfluenzainfection. Eachlungwaspreservedinformaldehyde- protein signatures in archeological samples, thereby allowing based solvent, adding to the difficulty of protein analysis due reconstructionofthetemporalandgeographicalspreadofdiseases. to formaldehyde’s crosslinking and denaturing effects. Protein As recovery from the majority of lung samples was relatively low extraction analysis was performed in a clean room and analyzed and not pathogen-specific, next steps will be to optimize extraction via liquid chromatography mass spectroscopy in order to identify protocols for pathogenic proteins in calcified samples. InvestigatingtheRoleofSpiralGanglionNeuronDegenerationinaTMPRSS3

Source:

Harvard / Harvard College | Pforzheimer House | Neuroscience | 2028 / 2025

Topics:

protein, sample, lung, tissue, signature, preserved, historical, majority, analyze, pathogen, analysi, extraction

Co-authors:

@julietgaron277 , @ashleyscott278 , @christinawarinner279