Understanding Glucose Metabolic Fate: Optimizing Acid Hydrolysis for LC-MS Detection of Water-Soluble Metabolites in Muscle Tissue

Authors:

Sirazam Munira, Bo Yuan, Sheng Tony Hui

Date Created:

2025-01-01

Course Title:

Professor:

Not specified

About Paper:

Glucose is a primary fuel source of energy for living organisms. mM to 5 M, and examined temperature’s impact on hydrolysis Using stable and radioactive glucose tracing in moderately fasted efficiency. We found that a concentration of 3 M and incubation wild-type mice, we found that 75% of circulating glucose is at 80 °C gave an ideal hydrolysis efficiency of over 95% for oxidized for energy supply, whereas the remaining 25% of glucose both acids. The acids, and/or the ammonium hydroxide used to is destined to various storage pools, including glycogen (thus neutralize the acids before LC-MS analysis, create matrix effects forming a glycogen-glucose futile cycle), protein, triglyceride, and suppress MS signal sensitivity. HCl exhibited on average and phospholipids. There are, however, additional water-soluble 17% greater ion suppression than HClO . This underscores the storagemoleculesthatremainunidentifiedinthemuscle, butnotin importance of using a calibration with matched matrix effect for the liver. We hypothesize that inthe muscles, oligomers of glucose accurate quantification. To account for another potential source of are an important storage form, which might be related to glycogen inaccuracy, we tested for heat-induced degradation of glucose in metabolism. To verify the chemical nature of these hypothesized the presence of strong acid and found it to be minimal. oligoglucose, we first aim to develop a hydrolysis method for Ongoing work will focus on transitioning to performing acid these molecules using glycogen as a benchmark, and then quantify hydrolysis of the oligoglucose isolate from the muscles. The the hydrolysis-released glucose using liquid chromatography-mass successful completion of this project may provide further insight spectrometry (LC-MS). into glucose metabolism. Wecomparedacidhydrolysisefficiencyofhydrochloric(HCl)and perchloric acid (HClO 4 across a range of concentrations from 50

Abstract:

Glucose is a primary fuel source of energy for living organisms. mM to 5 M, and examined temperature’s impact on hydrolysis Using stable and radioactive glucose tracing in moderately fasted efficiency. We found that a concentration of 3 M and incubation wild-type mice, we found that 75% of circulating glucose is at 80 °C gave an ideal hydrolysis efficiency of over 95% for oxidized for energy supply, whereas the remaining 25% of glucose both acids. The acids, and/or the ammonium hydroxide used to is destined to various storage pools, including glycogen (thus neutralize the acids before LC-MS analysis, create matrix effects forming a glycogen-glucose futile cycle), protein, triglyceride, and suppress MS signal sensitivity. HCl exhibited on average and phospholipids. There are, however, additional water-soluble 17% greater ion suppression than HClO . This underscores the storagemoleculesthatremainunidentifiedinthemuscle, butnotin importance of using a calibration with matched matrix effect for the liver. We hypothesize that inthe muscles, oligomers of glucose accurate quantification. To account for another potential source of are an important storage form, which might be related to glycogen inaccuracy, we tested for heat-induced degradation of glucose in metabolism. To verify the chemical nature of these hypothesized the presence of strong acid and found it to be minimal. oligoglucose, we first aim to develop a hydrolysis method for Ongoing work will focus on transitioning to performing acid these molecules using glycogen as a benchmark, and then quantify hydrolysis of the oligoglucose isolate from the muscles. The the hydrolysis-released glucose using liquid chromatography-mass successful completion of this project may provide further insight spectrometry (LC-MS). into glucose metabolism. Wecomparedacidhydrolysisefficiencyofhydrochloric(HCl)and perchloric acid (HClO 4 across a range of concentrations from 50

Source:

Harvard / Harvard College | Leverett House | Neuroscience | 2026 / 2025

Topics:

glucose, acid, hydrolysi, using, glycogen, muscle, found, water, source, energy, efficiency, concentration

Co-authors:

@sirazammunira336 , @boyuan337 , @shengtonyhui338