Memory Th2 Cells in Type 2 Inflammation in Asthma

Authors:

Zhixiao Yip, Mateus Lopes, Thao Nyugen, Andrew D. Luster

Date Created:

2025-01-01

Course Title:

Professor:

Not specified

About Paper:

Allergen-specific tissue-resident memory T helper 2 (Th2-Trm) Preliminary flow cytometry and immunofluorescence microscopy cells are known drivers of the chronic type 2 inflammation found data supported such an interaction between the two cells. As in asthma. Localizing to the peribronchial area in the lung, these CXCL16+ dendritic cells and CXCR6+ Th2-Trms were located cells are central to modulating the regional inflammation found at adjacent to lymphatic vessels, we also investigated the potential thosesites. CXCL16isachemokine(chemotacticcytokine)known role of the lymphatic endothelium in shaping the Th2-Trm cell to play a role in trafficking tissue-resident memory CD8+ T cells niche. Given Th2-Trm cells express IL-7R, we used spatial expressing the receptor CXCR6. As Th2-Trm cells also express transcriptomics to model lymphatic endothelium production of CXCR6, we hypothesized that unknown peri-bronchial sources of IL-7, a known survival factor mediating T-cell homeostasis, and CXCL16 orchestrate a Th2-Trm niche that persists through the investigate IL7-IL7R signaling as potentially forming another axis memory phase. We applied single-cell spatial transcriptomics on supporting Th2-Trm residency in the lung. Our findings were an in-house dataset of murine lung samples to assess the topology, reflected in a human spatial transcriptomics dataset of healthy and cellular niches, and signaling pathways around peri-bronchial asthmatic lung samples, suggesting similar mechanisms in humans Th2-Trm cells in the naive, acute-inflammatory, memory, and as well. Elucidating the mechanisms by which Th2-Trm are able rechallenged phases of a murine house dust mite (HDM) model of to reside in the lung between inflammatory episodes identifies asthma. WeidentifieddendriticcellsasapotentialCXCL16source potential targets for their depletion as a novel treatment for asthma. interacting with CXCR6+ Th2-Trm cells in peribronchial areas.

Abstract:

Allergen-specific tissue-resident memory T helper 2 (Th2-Trm) Preliminary flow cytometry and immunofluorescence microscopy cells are known drivers of the chronic type 2 inflammation found data supported such an interaction between the two cells. As in asthma. Localizing to the peribronchial area in the lung, these CXCL16+ dendritic cells and CXCR6+ Th2-Trms were located cells are central to modulating the regional inflammation found at adjacent to lymphatic vessels, we also investigated the potential thosesites. CXCL16isachemokine(chemotacticcytokine)known role of the lymphatic endothelium in shaping the Th2-Trm cell to play a role in trafficking tissue-resident memory CD8+ T cells niche. Given Th2-Trm cells express IL-7R, we used spatial expressing the receptor CXCR6. As Th2-Trm cells also express transcriptomics to model lymphatic endothelium production of CXCR6, we hypothesized that unknown peri-bronchial sources of IL-7, a known survival factor mediating T-cell homeostasis, and CXCL16 orchestrate a Th2-Trm niche that persists through the investigate IL7-IL7R signaling as potentially forming another axis memory phase. We applied single-cell spatial transcriptomics on supporting Th2-Trm residency in the lung. Our findings were an in-house dataset of murine lung samples to assess the topology, reflected in a human spatial transcriptomics dataset of healthy and cellular niches, and signaling pathways around peri-bronchial asthmatic lung samples, suggesting similar mechanisms in humans Th2-Trm cells in the naive, acute-inflammatory, memory, and as well. Elucidating the mechanisms by which Th2-Trm are able rechallenged phases of a murine house dust mite (HDM) model of to reside in the lung between inflammatory episodes identifies asthma. WeidentifieddendriticcellsasapotentialCXCL16source potential targets for their depletion as a novel treatment for asthma. interacting with CXCR6+ Th2-Trm cells in peribronchial areas.

Source:

Harvard / Harvard College | Lowell House | Mechanical Engineering | 2028 / 2025

Topics:

th2, cell, memory, lung, asthma, cxcr6, inflammation, known, lymphatic, niche, spatial, transcriptomic

Co-authors:

@zhixiaoyip393 , @mateuslopes394 , @thaonyugen395 , @andrewdluster396