Characterizing MSMEG 3748 : A Novel Cell Division Protein in Mycobacteria

Authors:

SangHoon Jung, Samuel Zinga, Wesley Parker, Eric Rubin

Date Created:

2025-01-01

Course Title:

Professor:

Not specified

About Paper:

Tuberculosis, caused by Mycobacterium tuberculosis, is the in similar phenotypes, validating a role of MSMEG 748 in3 world’s leading cause of death from a single infectious agent mycobacterial cell division. Furthermore, a minimum inhibitory despite it being a curable disease. The standard treatment involvesconcentration (MIC) assay of the knockout strain demonstrated a combination of antibiotics over several months, and novel drug that deletion of MSMEG causes a large susceptibility to Clp targets must be identified to shorten treatment period and combat protease inhibitor. In M. smegmatis, inhibition of Clp protease drug-resistant TB. Therefore it is important to investigate essential known to result in cell division defects, further supporting the proteins with uncharacterized functions in M. tuberculosis. One role of MSMEG 3748as a protein involved in mycobacterial cell suchproteinisRv1697. TheanalogofthisproteininM.smegmatis, division. MSMEG 3748, is not essential for growth in vitro and thus its Further research of MSMEG 3748 through growth curves, function can be closely studied. immunoprecipitation assays, MICs across a wider selection In this study, deletion of MSMEG 3748 via oligonucleotide of drugs, and imaging will unveil the finer workings of this recombineering resulted in elongated cells with more septa protein and its interactions. This research will provide a better and branching, indicative of a severe cell division defect. understanding of mycobacterial cell division and potentially Transcriptional knockdown of MSMEG 3748 through an identify a novel drug target to reinforce the treatment of TB. anhydrotetracycline (ATc)-inducible CRISPRi system resulted Exploring Refugee Experiences with Neurological Disorders through Photovoice Maab Karrar, Altaf Saadi Harvard College | Kirkland House | History and Science & Neuroscience | 2027 The number of forcibly displaced individuals worldwide has Refugee Health Providers and their Resettled Refugee Board. reached record levels due to ongoing wars and human rights violations. Over 51.1 million refugees and asylum seekers were We aim to recruit 30 English- and Spanish-speaking participants. To date, we have enrolled 22 participants, with n=7 having reported by the UN at the end of 2024. Due to experiences of completed the study and n=9 in ongoing groups. Enrolled trauma, like torture and interpersonal violence, they have greater vulnerability to poor health outcomes, including neurological participants have an average age of 36, are majority female conditions. Preliminary research from our lab has identified (63.6%), and represent 16 countries. Recruitment is ongoing. We have begun thematic analysis of photographs, session notes, and headache disorders, traumatic brain injury, and sleep disorders as transcripts. Preliminary themes include social isolation, dismissal the most common neurological conditions among U.S.-resettled of symptoms, workplace challenges, and the role of exercise, faith, refugees. This study explores refugees’ experiences with these conditions. and nature in coping. This project will culminate in online and in-person photo We employ Photovoice, a Community-Based Participatory Research method combining participant-led photography and exhibitions in community and academic spaces. The findings will groupdiscussion. Participantsusephotosandnarrativestorytelling inform care and policy recommendations. This project is the first ofthreephasesofalargerparentprojectaimingtotobuildcapacity to reflect on their lived experiences and challenges, which can be amongrefugeesparticipatinginneurologicresearchandtodevelop later used to address social and health-related issues through their dissemination. Our community partners include the Society of peer support for refugees with neurological disorders. 186 Summer Undergraduate Research in Global Health EMPOWER Youth Pilot Study Fajr Khan, Laura Bond, Vikram Patel Harvard College | Leverett House | Neuroscience | 2026 The Mental Health for All Lab’s EMPOWER initiative aims to questionnaire including the Acceptability of Intervention Measure, build the world’s mental health workforce. Through scaling up the Intervention Appropriateness Measure, the Feasibility of evidence-based psychosocial interventions, the lab aims to reduceIntervention Measure, and the System Usability Scale. Five semi- the global burden of mental disorders. The EMPOWER Youth structured focus group discussions were also conducted. The project focuses on mood and emotional disorders in youth by averagescoreonthepre-andpost-knowledgeassessmentincreased designing a digital training program for frontline workers who from78.85to83.73,butthischangewasnotstatisticallysignificant interact with adolescents in school or community-based settings. (p=0.081),potentiallyduetoahighbaselineknowledgeleveloran The training teaches a brief evidence-based transdiagnostic insufficiently challenging assessment. The questionnaire averages intervention emphasizing quick calming and problem-solving were high across all measures: feasibility = 4.5; acceptability = techniques. A single-arm pilot study assessed the feasibility, 4.5; appropriateness = 4.5 (0-5 scale), and system usability = 81.38 acceptability, and perceived effectiveness of the EMPOWER (0-100 scale). Evaluation of the competency assessments and Youth Digital Training program. A total of 52 frontline formal thematic content analysis of the focus groups is currently workers were recruited from the Ballmer Institute’s Graduate in progress. The preliminary results indicate that the knowledge MicrocredentialinChildBehavioralHealth(UniversityofOregon) assessment needs to be revised. Next steps include changing the and Davis Middle School (San Antonio, Texas). Participants digitaltrainingbasedonthefeedbackfromthepilotstudy,followed completed pre- and post-knowledge assessments, asynchronous byanimplementationstudywheretheeffectivenessoftheprogram competency assessments, and a dissemination and implementation for delivering interventions to youth is assessed. Impact of Real-Time Benefit Tool Notifications on Prescribing Behavior: Differences by Alert Type and Order Modification Rates Rina Matsunaga, Anna Doar Sinaiko Harvard College | Adams House | Economics | 2028 Real-time benefit tools (RTBTs), a feature of electronic health characteristics. The successful completion of the proposed project records (EHRs), provide clinicians with real-time out-of-pocket may indicate that alerts tied to administrative burden are perceived (OOP) price estimates for prescribed medications and their as more actionable than those related to OOP cost alone. The therapeutic equivalents. Cost-related underuse of medications findings may have important implications for optimizing RTBT account for preventable morbidity and mortality. However, alert design and improving clinical decision support systems. The little is known about how clinician responses vary with differentstudy may help identify which alert types are most effective in types of RTBT alerts, and whether physicians are more likely influencing prescribing behavior and where enhancements in alert to change their medication orders if they are alerted about priorrelevance and usability are needed. Because medical underuse authorization (PA) or coverage restrictions, in comparison to and adverse health outcomes are worse for minority patients alerts about high OOP costs. A cross-sectional study using and those who live in rural areas, understanding prescribing EHR prescribing data from University of Colorado Health from behavior is crucial because addressing gaps in cost information July 2019 to June 2024 was conducted. RTBT alerts were may reduce OOP spending and improve underuse. Furthermore, categorized by type: PA, coverage restriction, or cost estimate. as policymakers and payers increasingly focus on reforming PA The primary outcome was clinician response, categorized as practices, understanding how clinicians respond to RTBT alerts either modifying the original order or proceeding without change.mayinformeffortsinimprovingmedicationaccessandprioritizing We will compare rates of order modification across alert types actionable alerts, while minimizing the burden of too many EHR using linear regression, controlling for medication and patient alerts and alert fatigue. Assessing Engagement with Clinical Decision-Making Tools in African Medical Schools Dana Garibaldi Saenz, Safia Abou-Zamzam, Rebecca Weintraub Harvard College | Winthrop House | Human Developmental and Regenerative Biology & History and Science | 2027 Clinical Decision Support(CDS)tools are often used byhealthcare UpToDate user within these medical schools uses the platform 63 professionals in the United States to help them integrate the timespermonth. Thisdatahighlightstheexpansionofourdonation latest evidence-backed medical guidelines and treatments into program in underdeveloped countries as well as the frequent patient care. While these resources are essential to providing usage of UpToDate by participating African medical schools. quality healthcare, there is a financial barrier for medical schools,rther exploratory statistical analysis of this data will reveal key hospitals, and other institutions in low-income countries to accessinformation on the relationship between donation recipients in these tools. To address this need, the Global Health Delivery under-resourced settings and subsequent CDS usage. To extend Project (GHD) at the Brigham and Women’s Hospital Division the outreach of this project, GHD shares lessons learned with of Global Health Equity provides donation-based subscriptions to others in the field who are working to expand access to CDS these resources, including a leading CDS tool named UpToDate. tools and improve patient outcomes around the world. In all, this Our team has compiled UpToDate usage data across 65 African work will help support an increase in evidence-backed clinical medical schools with over 130,000 participating students, faculty, decision making worldwide, ultimately creating more equitable and residents. Current results suggest that on average, each global health systems. ASystematicReviewofInterventionstoImproveHPVVaccineUptakeAmong Adolescents Lily Song, Ingrid T. Katz Harvard College | Eliot House | Neuroscience | 2027 Human papillomavirus (HPV) is a prevalent sexually transmitted are being collected on study design, population characteristics, infection and a leading cause of several preventable cancers, intervention components, implementation strategies, and primary including cervical, anal, and oropharyngeal cancers. Although outcomes. Risk of bias assessments will be conducted using a safe and effective vaccine is available, HPV vaccination rates standardized tools. among adolescents remain below national targets, particularly While analysis is ongoing, preliminary review suggests that in underserved populations. This systematic review aims to multicomponent interventions—such as those combining provider evaluate the effectiveness of interventions designed to increase HPVvaccineuptakeamongadolescentsacrossschool,clinical,and education, reminder systems, and tailored communication—may community settings. be particularly effective. The review will help identify common facilitators and barriers across interventions and highlight gaps in Following PRISMA guidelines, we conducted a comprehensive existing research. search of peer-reviewed literature published between 2010 Future directions include completing data extraction and synthesis, and 2024. Eligible studies include randomized controlled assessing intervention effectiveness, and developing a framework trials, quasi-experimental studies, and observational studies that to guide future implementation efforts. These findings may inform assess interventions to improve HPV vaccine initiation and/or public health policy and help design more equitable, scalable completion. Two independent reviewers screened titles and abstracts, and we are currently in the data extraction phase. Data strategies to increase HPV vaccination coverage. Autographs Autographs Index A C Abdel-Hak, Marwa...............................84 Calixte, Jonora A.................................26 Acosta, Giselle ................................. 162 Carbajal, Brianna ............................... 141 Adonteng, Audric ................................ 84 Carpenter, Evan..................................45 Adri, Fabian ..................................... 85 Carter, Joseph....................................27 Agarwal, Isabelle ................................ 85 Chang, Michelle ................................. 91 Ahmad, Ayaan..................................159 Chapa, Gissell ................................... 91 Alghabra, Wissam .............................. 178 Chen, Alice......................................92 Chen, Sabrina....................................92 Allwood, Jovianna ............................... 24 Chen, Victoria ................................... 60 Amgen...........................................2 Cheng, Evelyn ................................... 69 An, David ....................................... 86 Cho, Esther......................................70 Anbarasu, Sarva ................................. 68 Cho, Kevin ...................................... 93 Ansley, Justin....................................24 Christensen, Anika Liv .......................... 184 Anthony, Matthew .............................. 148 Cimino, Anthony.................................12 Arian, M Aditta..................................68 Cleves, Laura....................................13 Arlanza, Aaron .................................. 12 Cole, Anisa.......................................4 Atieno, Faith.....................................86 Collardin, Lynn .................................. 80 Azam, Tahmid...................................87 Connally, Sawyer ............................... 163 Cooper, Tae Esperanza .......................... 149 Crawford, Kohl E.................................38 B Crider, Addison..................................93 Backman, Levi ................................. 174 Cross, Nyla......................................27 Curtis, Holly.....................................94 Bajomo, Ezekiel ................................. 87 Bakry, Wessal ................................... 44 Baly, Kaelen.....................................25 Bandla, Parnitha ................................. 88 D Banull, Alex.....................................69 DeJong, Brooke..................................94 Bentley, Elke .................................... 88 Di Tommaso, Leonardo..........................130 Bezwada, Hari ................................... 89 Dickerson, Kai...................................28 Bhargava, Arav .................................. 89 Diep, Michelle..................................179 Black, Sydney .................................. 178 Ding, Harry......................................13 Blackappl, Ludmila ............................. 179 Dixon, Zion .................................... 163 BLISS .......................................... 10 Dooley, Nia ..................................... 28 Bloom, Colin .................................... 44 DUBSP ......................................... 22 Bogroff, Malcolm...............................148 Duclas, Beverly..................................29 Borgman, Ella ................................... 90 Bowers, Aiden...................................90 Brandon, Ashton.................................25 Brooks, Skyy ................................... 162 E Bui, Khoi ...................................... 149 Ernst, Kaitlyn...................................170 Burnette, Hailey .............................. 26, 80 Eynon, Caleb .................................... 70 Eyre, Jaycelin....................................71 Hubbard, Jada ................................... 32 F I Fan, Baochan .................................... 95 Igwike, Emily .................................. 184 Farah, Hassan.....................................4 Ionides, Rita....................................151 Ferrari, Elena...................................144 Fleming, Ronni .................................. 45 Ford, Talia ...................................... 29 Freeman, Nia .................................... 30 J FUEL...........................................42 Jalloh, Misbahou.................................32 Fuentes, Diego A. Faria...........................71 Jarjoura, Jerome...................................8 Jensen, Wyatt...................................101 Ji, Joanne (Jae)...................................15 Jiang, Mira ..................................... 102 G Jones, Kirsti .................................... 102 Gabagat, Andrew..................................5 Jordan, Keon .................................... 33 Gadsby, Amélie..................................95 Joseph, Bryce....................................39 Garcia, Payton................................40, 72 Joseph-Lainez, Jacquelle..........................33 Garibaldi Saenz, Dana...........................187 Josephy, Matan .................................. 73 Garon, Juliet.....................................96 Jun, Kyra.......................................180 Gayle, Alexander ................................ 21 Jung, SangHoon ................................ 185 Gaytan, Juliette .................................. 52 Gelles, Carly.....................................96 George, Priscilla ................................. 46 Gong, Eric.......................................97 K Gorezi, Moana...................................97 Kang, Andrew ................................... 15 Grant, Vanessa...................................14 Karrar, Maab ................................... 185 Guide, Jyanne....................................30 Ke, Wan Ting .................................. 103 Gupta, Manya....................................98 Khan, Fajr......................................186 Killian, Ashley....................................5 Kim, Daniel .................................... 165 Kim, Elane ..................................... 103 H Kim, Eunice ..................................... 20 Ha, Samuel......................................98 Kim, Hannah .................................... 60 Haber, Isaac .................................... 156 Knight, Dalevyon ............................... 104 Hammond, Blake ................................ 52 Komeiji, Shane ................................. 104 Handulle, Yahya ................................. 99 Konar, Sandhya..................................53 Hashad, Akmal .................................. 99 Kouznetsov, Sophia ............................... 6 Hays, Claire .................................... 150 KRANIUM......................................58 Henderson, Tre’von .............................. 31 Herfort, Jamie .................................. 164 Hernández-Hernández, Mateo .................... 100 Herrera, Leonardo...............................150 L Herron, Guinn ................................... 31 L’Huillier, Nicolas .............................. 180 HIP.............................................50 Lai, Ericka ..................................... 105 Hokello, Selina ................................. 100 Laith, Hasan....................................170 Homma, Rin....................................101 Lambry Jr., Christopher...........................34 Hoogendoorn-Ecker, Kyler.......................164 Lamini, Francisca...............................105 Huang, Haoyang Harlan .........................151 Larson, Sonja...................................152 Huang, Max ..................................... 72 Le, William .................................... 106 Huang, Zihan (Harrison)..........................14 Lee, Kate.......................................106 192 INDEX Leifer, Emma...................................107 Norris, Elizabeth................................117 Leung, Kasie ................................... 107 Nwaishienyi, Silas ............................... 46 Li, Catherine ................................... 108 Nwakuche, Ifediora .............................. 35 Lian, Daniel J. .................................. 108 Lim, Kevin.....................................109 Lin, Mei ....................................... 109 Liu, Jona.......................................165 O Liu, Linda......................................110 Odutola, Sarah Rose.............................118 Liu, Michelle .................................... 73 Oji, Samuel N...................................118 Liu, Sophia.....................................142 Opria, Katherine .................................. 7 Liu, Ziyan.......................................53 Ouerfelli, Jude..................................119 Lord, Audrey ................................... 110 Lucini, Daniele ................................. 111 Ouyang, Nathan ................................ 119 P M Paley, Charlotte..................................19 Ma, Jessie......................................141 Palfrey, Emeline ................................ 120 Ma, Pei Yao Simon...............................61 Pan, Olivia ..................................... 120 Mahajan, Amann.................................16 Panetta, Giovanna...............................121 Mahmud, Lamisa ............................... 111 Pantham, Sairam................................121 Malague, Molly.................................112 Paraison, Chris-Ander ............................ 47 Martens, Laura ................................. 166 Park, Ian ....................................... 122 Martin, Amelie ................................. 112 Patel, Shikha ................................... 157 Matsunaga, Rina................................186 Pillai, Salini ..................................... 79 Mazumder, Aneesh..............................113 Ponce, Lucy .................................... 122 McKnight, Stoney................................34 PRIMO ......................................... 66 McNulty, Lily .................................. 156 PRISE .......................................... 82 Mei, Lauren ..................................... 16 Michaelides, Dora.................................6 Miller, Gage....................................113 Mills, Ivor.......................................74 R Mitchell, Nora ................................... 17 Rapping, Aaliyah ................................ 36 Mitragotri, Sitara................................114 REU...........................................146 Mohamed, Mohamed Haggag Farrag..............143 Rodrigo, Aryenne Ysabelle ...................... 123 Moore, Ian ...................................... 61 Rodriguez, Jeprika ................................ 7 Moore-Kosslow, Kaden..........................157 Romine-West, Keely ............................. 54 Morales, Devin ................................. 142 Moussa, Yasmine ............................... 114 Roper, Laine....................................181 Munira, Sirazam ................................ 115 Roy, Neel ...................................... 123 Rubio, Phoebe .................................... 8 Munson, Kennedi ................................ 35 Russ, Mia ...................................... 124 Murthy, Sonia .................................. 115 S N Sapers, Nayan .................................. 124 Nagumalli, Laasya ............................... 62 Scandelius, Carl..................................62 Nakhla, Charlotte ................................ 17 Schenenga, Mia.................................125 Nalbantoğlu, Sarp Ufuk...........................18 Schurman, Zoe ................................. 158 Nambiar, Viswajith ’Jith’ ........................ 116 SCION.........................................154 Nathwani, Zoe..................................116 Seay, Jayden.....................................36 Nguyen, Cecilia..................................18 Selip, Benjamin.................................158 Nguyen, Han ................................... 117 Serre, Rachel .................................... 74 Shamsi, Ahmad .................................. 54 Wagner, Romi .................................. 131 SHARP ........................................ 160 Wang, Aidan ................................... 132 Shen, Lucy ...................................... 55 Wang, Jessie....................................132 Shihab, Ahmed Fahim ............................ 75 Wang, Joseph...................................172 Shivaswamy, Aditya..............................55 Wang, Kevin ................................... 153 Silva, Matheus..................................125 Webber, Calvin .................................. 77 Smith, Marcus .................................. 126 Wells, Deborah .................................. 56 Smith, Zion......................................40 Wen, Justen .................................... 133 Song, Lily......................................187 Whittaker-Traw, Sylvia G. ........................ 20 Song, Yiding .................................... 63 Williams, A’Nyah................................38 Spencer, Liam .................................. 126 Wood, Lauren .................................. 172 Spiewak, Karolina................................47 Wright, Olivia .................................. 133 SPUDS ........................................ 168 Wu, Julianne ................................... 134 Sri-Ram, Alisha.................................127 Stevens, Elisabeth Maria Aleksandra...............48 Strand, Leela ................................... 127 Straw, Theresa..................................166 X Striker, Anthony ................................ 128 Xu, Angelina .................................... 77 SURF..........................................176 Xu, Eric.........................................64 SURGH........................................182 Xu, Lilly ........................................ 79 Swain, Sudhish ................................. 128 Xu, Rong.......................................134 Xue, Rebecca...................................135 T Tan, Luke ...................................... 129 Tang, Albert....................................129 Y Thomas, Gabriel ................................. 37 Yang, Audrey .................................. 173 Thompson, Asia ................................. 37 Yang, Danika...................................143 Thompson, Landen...............................39 Yang, Grace....................................173 Thompson, Leah ................................. 76 Yang, Masako .................................. 136 Todoran, Alexandru-Raul ......................... 63 Yang, Michelle ................................. 135 Tong, Eric......................................171 Ye, Gavin ....................................... 64 Topinio, Christian................................19 Yee, Sarah ..................................... 136 Truong, Ricky .................................. 171 Yin, Adrian......................................78 Yip, Zhixiao....................................137 Yoo, Claire.....................................137 Yoon, Joelle....................................138 U Yu, Veronica ................................... 138 Utgaard, Emerson...............................130 Yuan, Ammy ................................... 139 V Venkat, Vasumathi..............................152 Z Vidhun, Tanya..................................131 Zhang, Evan....................................139 Vinayaka, Umang................................76 Zhang, Nicholas ................................ 140 Zhang, Sofia....................................181 Zhang, Xinyi Christine .......................... 140 Zhou, Todd......................................65 W Zhu, Anthony....................................78

Abstract:

Tuberculosis, caused by Mycobacterium tuberculosis, is the in similar phenotypes, validating a role of MSMEG 748 in3 world’s leading cause of death from a single infectious agent mycobacterial cell division. Furthermore, a minimum inhibitory despite it being a curable disease. The standard treatment involvesconcentration (MIC) assay of the knockout strain demonstrated a combination of antibiotics over several months, and novel drug that deletion of MSMEG causes a large susceptibility to Clp targets must be identified to shorten treatment period and combat protease inhibitor. In M. smegmatis, inhibition of Clp protease drug-resistant TB. Therefore it is important to investigate essential known to result in cell division defects, further supporting the proteins with uncharacterized functions in M. tuberculosis. One role of MSMEG 3748as a protein involved in mycobacterial cell suchproteinisRv1697. TheanalogofthisproteininM.smegmatis, division. MSMEG 3748, is not essential for growth in vitro and thus its Further research of MSMEG 3748 through growth curves, function can be closely studied. immunoprecipitation assays, MICs across a wider selection In this study, deletion of MSMEG 3748 via oligonucleotide of drugs, and imaging will unveil the finer workings of this recombineering resulted in elongated cells with more septa protein and its interactions. This research will provide a better and branching, indicative of a severe cell division defect. understanding of mycobacterial cell division and potentially Transcriptional knockdown of MSMEG 3748 through an identify a novel drug target to reinforce the treatment of TB. anhydrotetracycline (ATc)-inducible CRISPRi system resulted Exploring Refugee Experiences with Neurological Disorders through Photovoice Maab Karrar, Altaf Saadi Harvard College | Kirkland House | History and Science & Neuroscience | 2027 The number of forcibly displaced individuals worldwide has Refugee Health Providers and their Resettled Refugee Board. reached record levels due to ongoing wars and human rights violations. Over 51.1 million refugees and asylum seekers were We aim to recruit 30 English- and Spanish-speaking participants. To date, we have enrolled 22 participants, with n=7 having reported by the UN at the end of 2024. Due to experiences of completed the study and n=9 in ongoing groups. Enrolled trauma, like torture and interpersonal violence, they have greater vulnerability to poor health outcomes, including neurological participants have an average age of 36, are majority female conditions. Preliminary research from our lab has identified (63.6%), and represent 16 countries. Recruitment is ongoing. We have begun thematic analysis of photographs, session notes, and headache disorders, traumatic brain injury, and sleep disorders as transcripts. Preliminary themes include social isolation, dismissal the most common neurological conditions among U.S.-resettled of symptoms, workplace challenges, and the role of exercise, faith, refugees. This study explores refugees’ experiences with these conditions. and nature in coping. This project will culminate in online and in-person photo We employ Photovoice, a Community-Based Participatory Research method combining participant-led photography and exhibitions in community and academic spaces. The findings will groupdiscussion. Participantsusephotosandnarrativestorytelling inform care and policy recommendations. This project is the first ofthreephasesofalargerparentprojectaimingtotobuildcapacity to reflect on their lived experiences and challenges, which can be amongrefugeesparticipatinginneurologicresearchandtodevelop later used to address social and health-related issues through their dissemination. Our community partners include the Society of peer support for refugees with neurological disorders. 186 Summer Undergraduate Research in Global Health EMPOWER Youth Pilot Study Fajr Khan, Laura Bond, Vikram Patel Harvard College | Leverett House | Neuroscience | 2026 The Mental Health for All Lab’s EMPOWER initiative aims to questionnaire including the Acceptability of Intervention Measure, build the world’s mental health workforce. Through scaling up the Intervention Appropriateness Measure, the Feasibility of evidence-based psychosocial interventions, the lab aims to reduceIntervention Measure, and the System Usability Scale. Five semi- the global burden of mental disorders. The EMPOWER Youth structured focus group discussions were also conducted. The project focuses on mood and emotional disorders in youth by averagescoreonthepre-andpost-knowledgeassessmentincreased designing a digital training program for frontline workers who from78.85to83.73,butthischangewasnotstatisticallysignificant interact with adolescents in school or community-based settings. (p=0.081),potentiallyduetoahighbaselineknowledgeleveloran The training teaches a brief evidence-based transdiagnostic insufficiently challenging assessment. The questionnaire averages intervention emphasizing quick calming and problem-solving were high across all measures: feasibility = 4.5; acceptability = techniques. A single-arm pilot study assessed the feasibility, 4.5; appropriateness = 4.5 (0-5 scale), and system usability = 81.38 acceptability, and perceived effectiveness of the EMPOWER (0-100 scale). Evaluation of the competency assessments and Youth Digital Training program. A total of 52 frontline formal thematic content analysis of the focus groups is currently workers were recruited from the Ballmer Institute’s Graduate in progress. The preliminary results indicate that the knowledge MicrocredentialinChildBehavioralHealth(UniversityofOregon) assessment needs to be revised. Next steps include changing the and Davis Middle School (San Antonio, Texas). Participants digitaltrainingbasedonthefeedbackfromthepilotstudy,followed completed pre- and post-knowledge assessments, asynchronous byanimplementationstudywheretheeffectivenessoftheprogram competency assessments, and a dissemination and implementation for delivering interventions to youth is assessed. Impact of Real-Time Benefit Tool Notifications on Prescribing Behavior: Differences by Alert Type and Order Modification Rates Rina Matsunaga, Anna Doar Sinaiko Harvard College | Adams House | Economics | 2028 Real-time benefit tools (RTBTs), a feature of electronic health characteristics. The successful completion of the proposed project records (EHRs), provide clinicians with real-time out-of-pocket may indicate that alerts tied to administrative burden are perceived (OOP) price estimates for prescribed medications and their as more actionable than those related to OOP cost alone. The therapeutic equivalents. Cost-related underuse of medications findings may have important implications for optimizing RTBT account for preventable morbidity and mortality. However, alert design and improving clinical decision support systems. The little is known about how clinician responses vary with differentstudy may help identify which alert types are most effective in types of RTBT alerts, and whether physicians are more likely influencing prescribing behavior and where enhancements in alert to change their medication orders if they are alerted about priorrelevance and usability are needed. Because medical underuse authorization (PA) or coverage restrictions, in comparison to and adverse health outcomes are worse for minority patients alerts about high OOP costs. A cross-sectional study using and those who live in rural areas, understanding prescribing EHR prescribing data from University of Colorado Health from behavior is crucial because addressing gaps in cost information July 2019 to June 2024 was conducted. RTBT alerts were may reduce OOP spending and improve underuse. Furthermore, categorized by type: PA, coverage restriction, or cost estimate. as policymakers and payers increasingly focus on reforming PA The primary outcome was clinician response, categorized as practices, understanding how clinicians respond to RTBT alerts either modifying the original order or proceeding without change.mayinformeffortsinimprovingmedicationaccessandprioritizing We will compare rates of order modification across alert types actionable alerts, while minimizing the burden of too many EHR using linear regression, controlling for medication and patient alerts and alert fatigue. Assessing Engagement with Clinical Decision-Making Tools in African Medical Schools Dana Garibaldi Saenz, Safia Abou-Zamzam, Rebecca Weintraub Harvard College | Winthrop House | Human Developmental and Regenerative Biology & History and Science | 2027 Clinical Decision Support(CDS)tools are often used byhealthcare UpToDate user within these medical schools uses the platform 63 professionals in the United States to help them integrate the timespermonth. Thisdatahighlightstheexpansionofourdonation latest evidence-backed medical guidelines and treatments into program in underdeveloped countries as well as the frequent patient care. While these resources are essential to providing usage of UpToDate by participating African medical schools. quality healthcare, there is a financial barrier for medical schools,rther exploratory statistical analysis of this data will reveal key hospitals, and other institutions in low-income countries to accessinformation on the relationship between donation recipients in these tools. To address this need, the Global Health Delivery under-resourced settings and subsequent CDS usage. To extend Project (GHD) at the Brigham and Women’s Hospital Division the outreach of this project, GHD shares lessons learned with of Global Health Equity provides donation-based subscriptions to others in the field who are working to expand access to CDS these resources, including a leading CDS tool named UpToDate. tools and improve patient outcomes around the world. In all, this Our team has compiled UpToDate usage data across 65 African work will help support an increase in evidence-backed clinical medical schools with over 130,000 participating students, faculty, decision making worldwide, ultimately creating more equitable and residents. Current results suggest that on average, each global health systems. ASystematicReviewofInterventionstoImproveHPVVaccineUptakeAmong Adolescents Lily Song, Ingrid T. Katz Harvard College | Eliot House | Neuroscience | 2027 Human papillomavirus (HPV) is a prevalent sexually transmitted are being collected on study design, population characteristics, infection and a leading cause of several preventable cancers, intervention components, implementation strategies, and primary including cervical, anal, and oropharyngeal cancers. Although outcomes. Risk of bias assessments will be conducted using a safe and effective vaccine is available, HPV vaccination rates standardized tools. among adolescents remain below national targets, particularly While analysis is ongoing, preliminary review suggests that in underserved populations. This systematic review aims to multicomponent interventions—such as those combining provider evaluate the effectiveness of interventions designed to increase HPVvaccineuptakeamongadolescentsacrossschool,clinical,and education, reminder systems, and tailored communication—may community settings. be particularly effective. The review will help identify common facilitators and barriers across interventions and highlight gaps in Following PRISMA guidelines, we conducted a comprehensive existing research. search of peer-reviewed literature published between 2010 Future directions include completing data extraction and synthesis, and 2024. Eligible studies include randomized controlled assessing intervention effectiveness, and developing a framework trials, quasi-experimental studies, and observational studies that to guide future implementation efforts. These findings may inform assess interventions to improve HPV vaccine initiation and/or public health policy and help design more equitable, scalable completion. Two independent reviewers screened titles and abstracts, and we are currently in the data extraction phase. Data strategies to increase HPV vaccination coverage. Autographs Autographs Index A C Abdel-Hak, Marwa...............................84 Calixte, Jonora A.................................26 Acosta, Giselle ................................. 162 Carbajal, Brianna ............................... 141 Adonteng, Audric ................................ 84 Carpenter, Evan..................................45 Adri, Fabian ..................................... 85 Carter, Joseph....................................27 Agarwal, Isabelle ................................ 85 Chang, Michelle ................................. 91 Ahmad, Ayaan..................................159 Chapa, Gissell ................................... 91 Alghabra, Wissam .............................. 178 Chen, Alice......................................92 Chen, Sabrina....................................92 Allwood, Jovianna ............................... 24 Chen, Victoria ................................... 60 Amgen...........................................2 Cheng, Evelyn ................................... 69 An, David ....................................... 86 Cho, Esther......................................70 Anbarasu, Sarva ................................. 68 Cho, Kevin ...................................... 93 Ansley, Justin....................................24 Christensen, Anika Liv .......................... 184 Anthony, Matthew .............................. 148 Cimino, Anthony.................................12 Arian, M Aditta..................................68 Cleves, Laura....................................13 Arlanza, Aaron .................................. 12 Cole, Anisa.......................................4 Atieno, Faith.....................................86 Collardin, Lynn .................................. 80 Azam, Tahmid...................................87 Connally, Sawyer ............................... 163 Cooper, Tae Esperanza .......................... 149 Crawford, Kohl E.................................38 B Crider, Addison..................................93 Backman, Levi ................................. 174 Cross, Nyla......................................27 Curtis, Holly.....................................94 Bajomo, Ezekiel ................................. 87 Bakry, Wessal ................................... 44 Baly, Kaelen.....................................25 Bandla, Parnitha ................................. 88 D Banull, Alex.....................................69 DeJong, Brooke..................................94 Bentley, Elke .................................... 88 Di Tommaso, Leonardo..........................130 Bezwada, Hari ................................... 89 Dickerson, Kai...................................28 Bhargava, Arav .................................. 89 Diep, Michelle..................................179 Black, Sydney .................................. 178 Ding, Harry......................................13 Blackappl, Ludmila ............................. 179 Dixon, Zion .................................... 163 BLISS .......................................... 10 Dooley, Nia ..................................... 28 Bloom, Colin .................................... 44 DUBSP ......................................... 22 Bogroff, Malcolm...............................148 Duclas, Beverly..................................29 Borgman, Ella ................................... 90 Bowers, Aiden...................................90 Brandon, Ashton.................................25 Brooks, Skyy ................................... 162 E Bui, Khoi ...................................... 149 Ernst, Kaitlyn...................................170 Burnette, Hailey .............................. 26, 80 Eynon, Caleb .................................... 70 Eyre, Jaycelin....................................71 Hubbard, Jada ................................... 32 F I Fan, Baochan .................................... 95 Igwike, Emily .................................. 184 Farah, Hassan.....................................4 Ionides, Rita....................................151 Ferrari, Elena...................................144 Fleming, Ronni .................................. 45 Ford, Talia ...................................... 29 Freeman, Nia .................................... 30 J FUEL...........................................42 Jalloh, Misbahou.................................32 Fuentes, Diego A. Faria...........................71 Jarjoura, Jerome...................................8 Jensen, Wyatt...................................101 Ji, Joanne (Jae)...................................15 Jiang, Mira ..................................... 102 G Jones, Kirsti .................................... 102 Gabagat, Andrew..................................5 Jordan, Keon .................................... 33 Gadsby, Amélie..................................95 Joseph, Bryce....................................39 Garcia, Payton................................40, 72 Joseph-Lainez, Jacquelle..........................33 Garibaldi Saenz, Dana...........................187 Josephy, Matan .................................. 73 Garon, Juliet.....................................96 Jun, Kyra.......................................180 Gayle, Alexander ................................ 21 Jung, SangHoon ................................ 185 Gaytan, Juliette .................................. 52 Gelles, Carly.....................................96 George, Priscilla ................................. 46 Gong, Eric.......................................97 K Gorezi, Moana...................................97 Kang, Andrew ................................... 15 Grant, Vanessa...................................14 Karrar, Maab ................................... 185 Guide, Jyanne....................................30 Ke, Wan Ting .................................. 103 Gupta, Manya....................................98 Khan, Fajr......................................186 Killian, Ashley....................................5 Kim, Daniel .................................... 165 Kim, Elane ..................................... 103 H Kim, Eunice ..................................... 20 Ha, Samuel......................................98 Kim, Hannah .................................... 60 Haber, Isaac .................................... 156 Knight, Dalevyon ............................... 104 Hammond, Blake ................................ 52 Komeiji, Shane ................................. 104 Handulle, Yahya ................................. 99 Konar, Sandhya..................................53 Hashad, Akmal .................................. 99 Kouznetsov, Sophia ............................... 6 Hays, Claire .................................... 150 KRANIUM......................................58 Henderson, Tre’von .............................. 31 Herfort, Jamie .................................. 164 Hernández-Hernández, Mateo .................... 100 Herrera, Leonardo...............................150 L Herron, Guinn ................................... 31 L’Huillier, Nicolas .............................. 180 HIP.............................................50 Lai, Ericka ..................................... 105 Hokello, Selina ................................. 100 Laith, Hasan....................................170 Homma, Rin....................................101 Lambry Jr., Christopher...........................34 Hoogendoorn-Ecker, Kyler.......................164 Lamini, Francisca...............................105 Huang, Haoyang Harlan .........................151 Larson, Sonja...................................152 Huang, Max ..................................... 72 Le, William .................................... 106 Huang, Zihan (Harrison)..........................14 Lee, Kate.......................................106 192 INDEX Leifer, Emma...................................107 Norris, Elizabeth................................117 Leung, Kasie ................................... 107 Nwaishienyi, Silas ............................... 46 Li, Catherine ................................... 108 Nwakuche, Ifediora .............................. 35 Lian, Daniel J. .................................. 108 Lim, Kevin.....................................109 Lin, Mei ....................................... 109 Liu, Jona.......................................165 O Liu, Linda......................................110 Odutola, Sarah Rose.............................118 Liu, Michelle .................................... 73 Oji, Samuel N...................................118 Liu, Sophia.....................................142 Opria, Katherine .................................. 7 Liu, Ziyan.......................................53 Ouerfelli, Jude..................................119 Lord, Audrey ................................... 110 Lucini, Daniele ................................. 111 Ouyang, Nathan ................................ 119 P M Paley, Charlotte..................................19 Ma, Jessie......................................141 Palfrey, Emeline ................................ 120 Ma, Pei Yao Simon...............................61 Pan, Olivia ..................................... 120 Mahajan, Amann.................................16 Panetta, Giovanna...............................121 Mahmud, Lamisa ............................... 111 Pantham, Sairam................................121 Malague, Molly.................................112 Paraison, Chris-Ander ............................ 47 Martens, Laura ................................. 166 Park, Ian ....................................... 122 Martin, Amelie ................................. 112 Patel, Shikha ................................... 157 Matsunaga, Rina................................186 Pillai, Salini ..................................... 79 Mazumder, Aneesh..............................113 Ponce, Lucy .................................... 122 McKnight, Stoney................................34 PRIMO ......................................... 66 McNulty, Lily .................................. 156 PRISE .......................................... 82 Mei, Lauren ..................................... 16 Michaelides, Dora.................................6 Miller, Gage....................................113 Mills, Ivor.......................................74 R Mitchell, Nora ................................... 17 Rapping, Aaliyah ................................ 36 Mitragotri, Sitara................................114 REU...........................................146 Mohamed, Mohamed Haggag Farrag..............143 Rodrigo, Aryenne Ysabelle ...................... 123 Moore, Ian ...................................... 61 Rodriguez, Jeprika ................................ 7 Moore-Kosslow, Kaden..........................157 Romine-West, Keely ............................. 54 Morales, Devin ................................. 142 Moussa, Yasmine ............................... 114 Roper, Laine....................................181 Munira, Sirazam ................................ 115 Roy, Neel ...................................... 123 Rubio, Phoebe .................................... 8 Munson, Kennedi ................................ 35 Russ, Mia ...................................... 124 Murthy, Sonia .................................. 115 S N Sapers, Nayan .................................. 124 Nagumalli, Laasya ............................... 62 Scandelius, Carl..................................62 Nakhla, Charlotte ................................ 17 Schenenga, Mia.................................125 Nalbantoğlu, Sarp Ufuk...........................18 Schurman, Zoe ................................. 158 Nambiar, Viswajith ’Jith’ ........................ 116 SCION.........................................154 Nathwani, Zoe..................................116 Seay, Jayden.....................................36 Nguyen, Cecilia..................................18 Selip, Benjamin.................................158 Nguyen, Han ................................... 117 Serre, Rachel .................................... 74 Shamsi, Ahmad .................................. 54 Wagner, Romi .................................. 131 SHARP ........................................ 160 Wang, Aidan ................................... 132 Shen, Lucy ...................................... 55 Wang, Jessie....................................132 Shihab, Ahmed Fahim ............................ 75 Wang, Joseph...................................172 Shivaswamy, Aditya..............................55 Wang, Kevin ................................... 153 Silva, Matheus..................................125 Webber, Calvin .................................. 77 Smith, Marcus .................................. 126 Wells, Deborah .................................. 56 Smith, Zion......................................40 Wen, Justen .................................... 133 Song, Lily......................................187 Whittaker-Traw, Sylvia G. ........................ 20 Song, Yiding .................................... 63 Williams, A’Nyah................................38 Spencer, Liam .................................. 126 Wood, Lauren .................................. 172 Spiewak, Karolina................................47 Wright, Olivia .................................. 133 SPUDS ........................................ 168 Wu, Julianne ................................... 134 Sri-Ram, Alisha.................................127 Stevens, Elisabeth Maria Aleksandra...............48 Strand, Leela ................................... 127 Straw, Theresa..................................166 X Striker, Anthony ................................ 128 Xu, Angelina .................................... 77 SURF..........................................176 Xu, Eric.........................................64 SURGH........................................182 Xu, Lilly ........................................ 79 Swain, Sudhish ................................. 128 Xu, Rong.......................................134 Xue, Rebecca...................................135 T Tan, Luke ...................................... 129 Tang, Albert....................................129 Y Thomas, Gabriel ................................. 37 Yang, Audrey .................................. 173 Thompson, Asia ................................. 37 Yang, Danika...................................143 Thompson, Landen...............................39 Yang, Grace....................................173 Thompson, Leah ................................. 76 Yang, Masako .................................. 136 Todoran, Alexandru-Raul ......................... 63 Yang, Michelle ................................. 135 Tong, Eric......................................171 Ye, Gavin ....................................... 64 Topinio, Christian................................19 Yee, Sarah ..................................... 136 Truong, Ricky .................................. 171 Yin, Adrian......................................78 Yip, Zhixiao....................................137 Yoo, Claire.....................................137 Yoon, Joelle....................................138 U Yu, Veronica ................................... 138 Utgaard, Emerson...............................130 Yuan, Ammy ................................... 139 V Venkat, Vasumathi..............................152 Z Vidhun, Tanya..................................131 Zhang, Evan....................................139 Vinayaka, Umang................................76 Zhang, Nicholas ................................ 140 Zhang, Sofia....................................181 Zhang, Xinyi Christine .......................... 140 Zhou, Todd......................................65 W Zhu, Anthony....................................78

Source:

Harvard / Harvard College | Winthrop House | Chemistry | 2028 / 2025

Topics:

health, alert, intervention, msmeg, tool, cell, division, refugee, medical, system, disorder, house

Co-authors:

@sanghoonjung464 , @samuelzinga465 , @wesleyparker466 , @ericrubin467