Characterization of Enteric Innervation in Colorectal Cancer

Authors:

Katherine Opria, Yisi Lu, Ruaidhr Jackson

Date Created:

2025-01-01

Course Title:

Professor:

Not specified

About Paper:

Increased enteric innervation in colorectal cancer (CRC) is the myenteric plexus, where the neuronal cell bodies reside, was associated with worse patient survival outcomes. My lab has further analyzed via whole-mount microscopy. We quantified + identified a key interaction between enteric neurons and CD8 + neural density in tumors using ImageJ, distinguished neurons from cells that may underlie this phenomenon: in response to CD8 T glial cells in the myenteric plexus, assessed neuronal activation cell-derived IFN-γ, enteric neurons upregulate PD-L1 and IL-18 via CFOS staining, and identified subtypes of enteric neurons binding protein, a soluble decoy receptor. Disrupting any part of innervating CRC tumors. We also confirmed that intrinsic enteric this pathway in vivo significantly reduced tumor burden in mice neuronal cell bodies in the myenteric plexus innervate the CRC with colitis-associated CRC. Despite these insights, the identity TME using an Alexa Fluor 488-labeled Cholera toxin subunit and extent of enteric neuronal innervation within the CRC tumor B (CTB-488) neuronal tracer strategy. This work advances our microenvironment (TME) remain poorly defined. To address understanding of the neural landscape in CRC and provides a this, we used the AOM/DSS, a murine model of colitis-associated framework for future studies on neuro-immune interactions in the CRC. Tumors were harvested, embedded in OCT, cryosectioned, TME. and immunostained with targeted antibody panels. Furthermore,

Abstract:

Increased enteric innervation in colorectal cancer (CRC) is the myenteric plexus, where the neuronal cell bodies reside, was associated with worse patient survival outcomes. My lab has further analyzed via whole-mount microscopy. We quantified + identified a key interaction between enteric neurons and CD8 + neural density in tumors using ImageJ, distinguished neurons from cells that may underlie this phenomenon: in response to CD8 T glial cells in the myenteric plexus, assessed neuronal activation cell-derived IFN-γ, enteric neurons upregulate PD-L1 and IL-18 via CFOS staining, and identified subtypes of enteric neurons binding protein, a soluble decoy receptor. Disrupting any part of innervating CRC tumors. We also confirmed that intrinsic enteric this pathway in vivo significantly reduced tumor burden in mice neuronal cell bodies in the myenteric plexus innervate the CRC with colitis-associated CRC. Despite these insights, the identity TME using an Alexa Fluor 488-labeled Cholera toxin subunit and extent of enteric neuronal innervation within the CRC tumor B (CTB-488) neuronal tracer strategy. This work advances our microenvironment (TME) remain poorly defined. To address understanding of the neural landscape in CRC and provides a this, we used the AOM/DSS, a murine model of colitis-associated framework for future studies on neuro-immune interactions in the CRC. Tumors were harvested, embedded in OCT, cryosectioned, TME. and immunostained with targeted antibody panels. Furthermore,

Source:

Harvard / Harvard-Amgen Scholars Program 7 / 2025

Topics:

enteric, crc, neuronal, cell, tumor, neuron, innervation, myenteric, plexu, tme, colorectal, cancer

Co-authors:

@katherineopria116 , @yisilu117 , @ruaidhrjackson118