Sofia
Torre
Investigating PDGFRα-dependent OPC Density in Early Development Myelination is a fundamental process in vertebrate nervous system development, insulating axons to enable rapid and efficient neuronal signaling. Oligodendrocytes, which develop from oligodendrocyte precursor cells (OPCs), mediate this process in the central nervous system (CNS). Platelet-derived
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Sofia Torre
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growth factor receptor α (PDGFRα) signaling is known to regulate the proliferation, migration, and survival of OPCs. However, it is not known whether PDGFRa signaling is the sole driver of these mechanisms during early nervous system development. To characterize this, we generated a transgenic mouse model, Olig2-Cre(+/-); PDGFRα(fl/fl), in which PDGFRα is conditionally knocked out from the oligodendrocyte lineage, and used immunohistochemistry and fluorescence imaging analysis to reveal whether proper OPC density occurs in the absence of PDGFRa signaling in OPCs. We examined OPCs in the hippocampus, striatum, cerebellum, corpus callosum, and cortex of conditional PDGFRα KOs (PDGFRa cKOs) and PDGFRa floxed only littermate controls (PDGFRa CTL) at postnatal day 7 (P7). This work provides important insights into the mechanisms that underlie proper brain development, and future studies will focus on examining more regional differences and better characterizing the proliferation of OPC populations studied. 105 Soyu Hong:
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Brown / Summer Research Assistantship in Biomedical Sciences
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Sofia Torre