Authors:

Tata (Chaipat) Tirapongprasert

Date Created:

Not specified

Course Title:

Professor:

Not specified

About Paper:

Studying early mammalian development and pluripotency in vitro requires the generation of embryo-derived cell lines. Mouse embryonic stem cells (mESCs) are derived from the naïve epiblast (Epi), which arises at 4.5 days post-fertilization, and model early embryonic development and gastrulation when cultured under specific conditions. In vitro, mESCs can be differentiated into epiblast-like cells (EpiLCs) and epiblast stem cells (EpiSCs), which parallel the formative Epi at E5.5 and the primed Epi at E6.5 in vivo. SOX2 is a transcription factor that has been implicated in the transition of mESCs from the naïve pluripotent state and may serve as an important regulator of embryonic Epi differentiation. This project seeks to validate preexisting methods for generating pluripotent stem cell (PSC) states and organoid models in vitro using mESCs. Immunostaining and fluorescence for lineage-specific transcription factors was used to confirm successful recapitulation of the embryonic PSC states. A SOX2 degron allele model was used to degrade endogenous SOX2 protein to further elucidate the influence of SOX2 as a director of mESC pluripotency transitions in vitro.

Source:

Columbia / Astrophysics / 2027

Topics:

No topics listed

Co-authors:

Tata (Chaipat) Tirapongprasert