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Novel Functional Characterization of Alternative Splicing of TCAIM in Breast Cancer

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Allison Chou, Yong Wei, Yibin Kang Cancer is one of the world's leading causes of death, with female breast cancer as the current leading cause of new diagnoses. Alternative splicing, a process that affects most human genes, is often dysregulated in cancer. In recent years despite the efforts to quantify alternative splicing in cancer patients using established transcriptome data, few studies have functionally characterized these identified, potentially important alternative splicing events in cancer progression. Further characterization of these events will reveal novel mechanisms of metastasis regulation and develop novel diagnostic markers and therapeutic options. Our preliminary computational work has shown that TCAIM (T cell activation inhibitor, mitochondria) is alternatively spliced in human breast cancer. The different isoforms of TCAIM are differentially expressed in tumor vs normal and among major breast cancer subtypes, indicating potential contributions to breast cancer tumorigenesis and/or cancer progression. We propose to characterize the functions of the short and long variants of TCAIM in breast cancer tumorigenesis, tumor progression and metastasis using lentiviral knockdown and overexpression systems. The study may determine the potential role of TCAIM and its splicing regulation in tumorigenesis and tumor progression.

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Princeton

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Novel Functional Characterization of Alternative Splicing of TCAIM in Breast Cancer