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Senior Thesis Fund.

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Abstract Maria Morales-Salgado, Coleen Murphy Cell identification and characterization has led to several scientific discoveries and a comprehensive understanding of many types of cells across several organisms. The well-developed Single-cell RNA sequencing (scRNA-seq) technique has been central in achieving such identification of cells and the development of the human cell atlas. However, scRNA-seq has only been able to identify types of cells in their average states without specificity to the state of each individual cell. For Caenorhabditis elegans, scRNA-seq was used to identify neuron-enriched genes. However, as C. elegans contain over 300 types of neurons, it is of interest to characterize the state of each individual neuron type. With this goal in mind, this research focuses on creating a scRNA-seq protocol to identify which C. Elegans neuron types change for phenotypically different worms or mutant worms. Fluorescence-activated Cell Sorting (FACS) aided this process by differentiating the nuclei from neurons within the worms. To identify the nuclei specific to neurons, a green fluorescent protein worm strain was created which tagged all of the neurons of the worms. To get the highest number of fluorescent worms for the FACS machine, the strain was fully integrated such that all of the worms had fluorescents. After passing the sample through the FACS machine, each individual cell-type and transcriptional state classifications would be made, specifying how the expression of each neuron type changes for mutant worms.

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Princeton

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Senior Thesis Fund.