Authors:

Deepika Goyal

Date Created:

Not specified

Course Title:

Professor:

Not specified

About Paper:

MicroRNAs (miRNAs) are small, 19-23 nucleotide-long noncoding RNA molecules that regulate gene expression at the post-transcriptional level. Their expression varies significantly in diseases such as cancer and autoimmune disorders compared to normal cells. MicroRNA 18a and 19a have differential expressions in canine mammary tumor cell lines and the exosomes released by these cells compared to normal epithelial cells. Using miRDB, we have identified miR-18a and miR-19a as potential regulators of estrogen receptor 1 (ESR1) gene expression. Like human breast cancer, canine mammary tumors can also be classified into four types depending on the presence or absence of Estrogen receptor, Progesterone receptor, and HER2. In this study, we are investigating the role of miR-18a and miR-19a in regulating Estrogen receptor 1 expression in the canine mammary tumor lines. Luciferase reporter assays confirmed that these miRNAs directly target the gene ESR1, leading to its differential expression in cancerous versus normal cells. Functional analyses revealed that miRNA-mediated suppression of ESR1 expression influenced key cellular processes, such as proliferation, migration, and invasion. These findings suggest that miRNAs targeting the ESR1 mRNA may serve as potential biomarkers or therapeutic targets in breast cancer. Future studies will explore the broader implications of this regulatory mechanism in tumor progression and treatment strategies.

Source:

Auburn University / College of Veterinary Medicine / 2025

Topics:

No topics listed

Co-authors:

Deepika Goyal