Authors:

Kennedy Moes

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About Paper:

TSpinal cord injury (SCI) affects over 299,000 patients globally. However, to date there is no treatment available that can promote locomotor recovery after SCI. In this project, we aim to develop a clinically translatable injectable hydrogel that can provide pro-regenerative cues for promoting axonal regeneration. Decellularized human sciatic nerve (dHSN) based allograft do exists and have shown potential in peripheral nerve regeneration. However, fabrication of injectable hydrogel using dHSN is still a challenge. In this proposal, human sciatic nerves were obtained from NDRI tissue bank. The nerves were then decellularized modifying our previous protocol for rat peripheral nerves, in brief adding a delipidation step and increasing the time of each wash. To test the efficiency of the decellularized process, DAPI stain was performed on resulting decellularized nerve segment and compared with fresh nerve. Confocal imaging revealed absence of DAPI staining in decellularized nerves, demonstrating devoid of nuclei presence in decellularized nerves. In future, we plan to perform Oil Red O staining (for estimation of lipid remnants), extracellular marker staining (Collagen I, Collagen IV and laminin), and analyze the potential of decellularized human peripheral nerve matrix to digest and form injectable formulation. We anticipate that developing injectable formulation using dHSN is a clinical viable biomaterial- based approach for SCI regeneration in patients.

Source:

University of Florida / Kennedy Moes, Gopal Agarwal, Christine E. Schmidt / 2023

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Co-authors:

Kennedy Moes