Authors:

Kennedy Moes

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Spinal cord injury (SCI) can result in permanent loss of sensory and motor functions. According to the World Health Organization, it is estimated that 15.4 million peopl e were living with SCI globally in 2021. Currently, there are no FDA-approv ed treatments for locomotor recovery after SCI. Decellularized human peripheral ner ves have shown the potential to promote axonal regeneration in peripheral nerve disea ses. However, developing an injectable hydrogel using decellularized human nerves is st ill a challenge due to the presence of lipids. In this study, we developed an inje ctable hydrogel using decellularized human sciatic nerve (iHPN). Developing an inj ectable hydrogel allows us to develop a minimum invasiveness hydrogel solution tha t can be directly administered at the site of injury. Furthermore, the presenc e of pro-regenerative cues like Collagen I, laminin, and various growth factors ca n promote axonal regeneration in the injured spinal cord. In the current study, human n erves were decellularized modifying the Hudson protocol previously published by o ur lab, and delipidated using various organic solvents. The nerves were then digested and neutralized to p.H. 7.4 and to match the CNS native mechanical streng th genipin, a natural crosslinker was used. The resultant hydrogels exhibited inj ectability and mechanical strength that can support neuronal cell growth. Overal l, iHPN has the potential to promote axonal regeneration in the injured spinal cord and can also be used clinically as a delivery vehicle for SCI regeneration.

Source:

University of Florida / Kennedy Moes, Gopal Agarwal, Christine E. Schmidt / 2024

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Co-authors:

Kennedy Moes