Authors:

Tapley Sorenson

Date Created:

Not specified

Course Title:

Professor:

Not specified

About Paper:

Mouse Model Activity in the noradrenergic system is critical for modulating an animal's levels of attention and arousal while awake, though far less research has been carried out by examining noradrenergic activity during sleep.. While the understanding of the functional organization of the noradrenergic system is still evolving, it has been established that it is capable of broadcasting information both globally, to all the regions of the central nervous system that it innervates, as well as locally, only to specific destinations. Additionally, female animals have been shown to experience systemic disruptions to their sleep due to hormonal fluctuations when compared to males. We will use two-photon microscopy and genetically encoded calcium indicators 328 UNIVERSITY OF OREGON • 2024 UNDERGRADUATE RESEARCH SYMPOSIUM (GECI) to record noradrenergic axons across brain states throughout the cerebral cortex, categorizing sleep states with EMG, EEG, and pupillometry. We will retrospectively compare the results to determine whether there is a significant difference in noradrenergic synchrony across sleep states by biological sex. Two-photon microscopy allows for simultaneous measurement of activity between individual neurons, such that we can assess the heterogeneity of sleep patterns across multiple cortical regions of interest. Sleep patterns are generally conceptualized as broad oscillatory signals, but that is based on global recordings suggesting that there could exist a greater variance in activity across localized brain regions.

Source:

University of Oregon / 2024

Topics:

No topics listed

Co-authors:

Tapley Sorenson