Authors:

Jillian Vogel

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The Three-spined Stickleback (Gasterosteus aculeatus) is widely used in laboratories studying evolutionary genetics, evolutionary biology, and population genetics due to its adaptability and well-characterized genome. Preserving reproductive material from model organisms is vital for research continuity, genetic resource banking, and conservation, but a reliable method has yet to be established for this species specifically. Because cryopreservation efficacy varies between species, several parameters were tested to identify an optimal protocol tailored to the Three-spined Stickleback. This study aimed to develop a reproducible method for whole testis vitrification, an approach with potential to improve long-term sperm storage and recovery. To optimize cryopreservation, we tested multiple vitrification procedures prior to cryogenic freezing, including variation in cryoprotectant type, concentration, and incubation duration. Post-thaw sperm quality was assessed using three key characteristics: morphology, fertilization capacity, and DNA fragmentation. Our findings contribute to the development of standardized protocols for germline preservation in fish, supporting future research, aquaculture, and biodiversity conservation efforts. Volz, Skylar See "Knudsvig, Olivia" From Co-Management to Land Back: (Re)Imagining Mesa Verde National Park 315 UNIVERSITY OF OREGON • 2025 UNDERGRADUATE RESEARCH SYMPOSIUM TABLE OF CONTENTS

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University of Oregon / 2025

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Co-authors:

Jillian Vogel