Authors:

Payton Taylor

Date Created:

Not specified

Course Title:

Professor:

Not specified

About Paper:

Phenolic acid degrading endophytes colonize the inner tissues of poplar and influence the rhizosphere environment by causing the soil priming effect and may be beneficial to the plant by fixing nitrogen. This study aims to explore the aromatic-degrading endophytic bacteria in three different transgenic poplar, growing at Luger Farm, which produces varying amount of phenolic monolignols derived from sinapyl (S) and coniferyl alcohols (G). We hypothesize that the composition of aromatics between high S:G (F5H85 ) and low S:G transgenic lineages (0998-45) will cause variation in the endophyte populations. Samples were collected from a high S line, a low S line, and a wild type poplar (WT 717). We selectively enriched for phenolic acid- degrading bacteria using minimal media containing Benzoic Acid or Hydroxybenzoic Acid as sole carbon sources. Additional enrichment culturing was conducted with or without a chemical nitrogen source (to select for N-fixing bacteria) and with or without Cycloheximide, a fungicide, to select for or against fungi. Enrichment culturing was performed on media with Hydroxybenzoic Acid or Benzoic Acid followed by a dilution series to estimate the number of active cells in each sample. The optical density was collected over time to monitor microbial growth and observe differences between treatment groups. The ODs of the wild type poplar were highest when cultured in Hydroxybenzoic Acid and lowest when cultured in media containing Nitrogen and Benzoic Acid. The two mutant poplars were only cultured in media containing Nitrogen, but both had the highest average optical densities in media containing Hydroxybenzoic Acid.

Source:

Purdue University / 2023

Topics:

No topics listed

Co-authors:

Payton Taylor