Authors:

Mariana Guzman Sanchez

Date Created:

Not specified

Course Title:

Professor:

Not specified

About Paper:

Gut microbial interactions are influenced by the variety of dietary substrates available, as different microbes show variations in substrate preferences. By understanding these preferences, it is possible to selectively stimulate growth and activity of beneficial gut microbes. Inulin-type fructans and fructooligosaccharides are nondigestible, highly fermentable carbohydrates that can be hydrolyzed by a multitude of microorganisms. To explore the competition mechanisms related to complex polysaccharide degradation, a modeling approach is proposed. This research aims to study the consumption of inulins as carbon sources by Klebsiella pneunomiae, Escherichia coli and Bifidobacterium dentium to build a mathematical model that predicts and simulates microbial interactions. Monoculture batch fermentations were conducted under aerobic and anaerobic conditions using FB and M9 buffer supplemented with glucose, fructose, sucrose, kestose, and CLR at 0.4% concentration. Cultures were incubated for 8 hours at 37ºC, and samples were collected at 1-hour intervals. Bacterial growth was assessed by measuring optical density at 600 nm, microbial biomass was determined by dry weight through oven drying, and substrate concentration was analyzed using High- Performance Liquid Chromatography. Results revealed significant differences in maximum growth rates and yields among the various substrates investigated, as well as among the bacteria tested. Notably, the utilization of sucrose led to higher growth rates and yields, outperforming glucose and fructose. These differences are linked to substrate preferences, which are based upon degradation capabilities, metabolic pathways, and enzyme expression patterns. By comprehending the individual growth kinetics of bacteria, it is possible to forecast the outcomes of competition in future experiments.

Source:

Purdue University / 2023

Topics:

No topics listed

Co-authors:

Mariana Guzman Sanchez