Authors:

Jacob Stallman

Date Created:

Not specified

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About Paper:

Spinal cord injuries are devastating and can lead to many complications including allodynia, hyperalgesia, spasticity, and loss of sensory and motor function. Currently, there are no treatment plans that can successfully repair spinal cord injuries, so primary care providers are forced to resort to treating symptoms. A promising research avenue for the treatment of spinal cord injuries is neural progenitor cell (NPC) transplantation. This form of stem cell therapy has been shown to integrate into the host's nervous system and improve sensory function in mice and primate models, but no one has investigated exactly which subtypes of sensory neurons are forming connections with the neural progenitor cell graft. To do this, we used Rabies helper/Syn1-cre grafts in C4 dorsal column lesions and monosynaptic rabies tracing. By colocalizing rabies positive neurons with other biomarkers visualized through fluorescence imaging and immunohistochemistry we will be able to see which specific subtypes are forming these connections. Additionally, by performing this analysis at every level of the spinal cord, we will be able to visualize the extent of connections these grafts can make. These results will help gain a greater knowledge of how sensory function improves after NPC transplantation and the graft cytoarchitecture from which it originates. Poster #11 Characterizing Effects of Anterior-Posterior Identity on Neural Progenitor Cell Graft Integration after Spinal Cord Injury Joseph Hoppe

Source:

Texas A&M University / 2023

Topics:

No topics listed

Co-authors:

Jacob Stallman