Authors:

Joseph Hoppe

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Transplanting neural progenitor cells (NPCs) into sites of spinal cord injury (SCI) is a promising approach at rebuilding complex neuronal connections and potentially restoring lost neurological function. Studies have shown that the broad regional identity of transplanted NPCs "" for example, brain versus spinal cord identity "" has a significant impact on graft integration into SCI sites in rodent models. However, no studies have looked specifically at how NPCs derived from anterior versus posterior embryonic spinal cord regions might differentially integrate into the injured spinal cord. In this study, we aimed to understand whether NPCs of anterior or posterior tissue identity exhibit differences in neurogenesis and gliogenesis, or differences in integration with existing neurons within the injured nervous system. We performed dorsal column lesions at the C5 spinal cord level in adult wild-type mice. NPCs were obtained from either the anterior or posterior regions of GFP+ embryonic (E12.5) mouse spinal cords and transplanted into the injured adult SCI mouse models. Grafts were given 8 weeks post-transplantation to mature and integrate in vivo, then mice were sacrificed. The spinal cord tissue was then cryo-sectioned transversely, and the sections underwent immunohistochemistry. Image analysis of the graft and surrounding tissue using FIJI software was performed. As predicted, we did not observe any significant differences in graft volume, Sox9+ astrocyte density, neuronal density, or axon outgrowth between the two groups. Currently, quantitative PCR is being performed to further analyze gene expression differences in the anterior and posterior regions of embryonic spinal cords. Poster #12 Counterfactual Thinking, the ABC Model, and Healthy Eating Intentions Meagan C. Hodges

Source:

Texas A&M University / 2023

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Co-authors:

Joseph Hoppe