Authors:

Archisha Biswas

Date Created:

Not specified

Course Title:

Professor:

Not specified

About Paper:

Bacterial tolerance to antibiotics is becoming a growing issue, as bacteria are adapting to persist despite treatment. Previous research has shown that outer membrane proteins called porins are associated with cellular permeability, allowing nutrients and antibiotics into the cell. In this study, an investigation into the mass transport of antibiotics will be characterized via porin channels. 10 clinical E. Coli strains from cancer patients were imaged, half of which display antibiotic tolerant behavior. Ethidium bromide (EtBr), mimicking antibiotics, was visualized in cells using fluorescence microscopy while phenylalanine-arginine β-naphthylamide (PAβN) blocked the efflux out of the bacteria. The resulting intensity of the fluorescence in cells were then analyzed. MATLAB code will be used to analyze images and properly characterize the uptake of EtBr in different strains grown with Meropenem, a broad-spectrum antibiotic commonly used for serious gram-negative bacterial infections, often used when other antibiotics are ineffective. Understanding the differences in porin function between strains, in the presence of Meropenem, will provide critical knowledge regarding how 5 out of the 10 clinical strains are tolerating Meropenem. Additionally, several Fluorescence-Activated Cell Sorting (FACS) and Plate Reader experiments were conducted using common antibiotics such as Dexamethasone, Methotrexate Sodium, Promethazine, Ciprofloxacin, and Rifampicin compounds to better understand tolerance towards antibiotics. Poster #4 Cartilage-Capped Regenerative Osteochondral Plug Andrew Haney

Source:

Texas A&M University / 2025

Topics:

No topics listed

Co-authors:

Archisha Biswas