Authors:

Nelly Escalante

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Natural Resistance Associated Macrophage Protein 1 (NRAMP1) is a metal ion transporter expressed in phagocytic cells such as macrophages, neutrophils, and dendritic cells. NRAMP1 deficiency has been associated with greater susceptibility to infections by intracellular pathogens such as Salmonella enterica serotype Typhimurium (S. ser. Typhimurium). However, the mechanism by which NRAMP1 protects against pathogens and infection has not been described. We propose that the main antimicrobial mechanism of NRAMP1 is to alter cytosolic iron concentrations to cause metabolic reprogramming of the cell, which includes the production of different cytokines. Using bone marrow-derived macrophages (BMDM), we aim to characterize differences in inflammatory cytokine signatures between NRAMP1 functional and deficient macrophages. Additionally, we aim to investigate whether the polarization state of macrophages (M0, M1, M2) influences the effect of NRAMP1 function on the production of different cytokine signatures. We are using qPCR to measure the expression of several inflammatory cytokines of S. ser. Typhimurium infected BMDM at different points of infection. The results of these experiments will help us further understand the role of NRAMP1 in limiting S. ser. Typhimurium infection and observe which macrophage polarization states exhibit greater NRAMP1 activity. Immigrant Flights or Immigrant Rights?: A comparative analysis of political party rhetoric and immigration rates Julia Estrada

Source:

UC Davis / MED: Medical Microbiology & Imm / 2023

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Co-authors:

Nelly Escalante