Timothy
Gutierrez
Sponsor: Isaya Kisekka, Ph.D. Biological & Ag Engineering This paper examines a novel Radio Frequency (RF) soil moisture sensor that uses delay time in radio wave propagation through soil to derive the dielectric constant of the soil and consequently the soil volumetric water content. Potential benefits of this sensor include resistance to high salinity levels and air gaps due to imperfect installation, which affect some currently available soil moisture sensors. The experimental setup for the sensor calibration includes varied soil volumetric water content (θ) and salinity levels (dS/m) over two major California soils and glass beads as a laboratory standard. Additionally, the novel sensor is compared with and without a simulated air gap, created using polyvinyl chloride electrical tape wrapped around the sensor prongs. A time domain reflectometry (TDR) sensor and gravimetric measurements of θ were used to validate the sensor. Results showed a high correlation between delay time and soil water content regardless of salinity level, while the presence of an air gap presented a slight reduction in RMSE. Soil-specific calibration was also found to reduce the error for all soils. Results indicate that the sensor performs as intended and would aid regions containing saline soils or soils that are subjected to intermittent drought. Ibuprofen Alters Cellular Function Correlated with Oxidative Stress in Liver Tissue and Cardiac Cell Lines
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Timothy Gutierrez
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Oxidative stress describes the imbalance in production of reactive oxygen species (ROS) and antioxidants. Prolonged oxidative stress is linked with pathological conditions. The mechanisms by which ibuprofen causes its side effects might be explained through induction of oxidative stress. Therefore, I examined markers of oxidative stress in mice treated with ibuprofen. Control mice were provided with water. Mice in the experimental group were administered ibuprofen in drinking water at a concentration of 100 mg/kg/day for seven days. This dosage approximates human consumption of 486 mg/day. Lipid Peroxidation Assay, with a control group of n=4 and an experimental group of n=5 was performed on a 96-well fluorometric plate to measure lipid peroxidation in liver tissue. Glucose-1-Phosphate Assay with a control group of n=5 and an experimental group of n=5 was performed on a colorimetric plate to measure glucose- 1-phosphate levels. H9c2 cells were harvested and used for ROS assays. In the ROS intensity assay, cells in the experimental group were treated with a dosage of 400μM (micromolar) ibuprofen. Equal amounts of protein (20μg) were separated on 4-20% TGX precast gels and transferred to membrane. Across all markers, the Ibuprofen-treated groups displayed increased markers of oxidative stress thus supporting the hypothesis. An Ecological Model of Resources and Services for Navigating Pregnancy After Sexual Assault Stephanie Ha
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UC Davis / MED: Physiology & Membrane Biol / 2023
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Timothy Gutierrez