Brian
Luong
Sponsor: Pamela Lein, Ph.D. VM: Molecular Bio Sciences Acute intoxication with cholinesterase-inhibiting organophosphorus (OP) pesticides can trigger life-threatening seizures associated with chronic neurological complications. Compromised blood-brain barrier (BBB) integrity has been proposed as a pathogenic mechanism linking the acute toxicity of OPs to chronic adverse neurological outcomes. We have shown that acute OP intoxication causes BBB impairment in a rat model; however, whether BBB breakdown is the result of the seizure activity or toxic effects of OPs not related to seizures is unknown. To evaluate the seizure-dependency of BBB impairment post-OP intoxication, we pharmacologically inhibited seizures triggered by acute intoxication with the OP, diisopropylfluorophosphate (DFP). Adult male Sprague-Dawley rats (n=24) were surgically implanted with electroencephalography (EEG) telemetry devices to monitor seizure activity. Animals were administered either vehicle or the anti-seizure compound midazolam (MDZ, 3 mg/kg, s.c.) 30 min prior to DFP exposure (3.75 mg/kg). Brain tissues were collected 1 day post-exposure. EEG analysis confirmed that midazolam pretreatment suppressed DFP-induced seizure activity. Our next steps will be to compare the extent of BBB leakage post-DFP intoxication using serum albumin immunolabeling in animals with and without MDZ pretreatment. This work supported by the UC Davis CounterACT program (U54 NS127758). Identification of a Threshold Toe Arm Index to Predict Wound Healing in Patients Undergoing Vascular Intervention
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Brian Luong
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The Society for Vascular Surgery (SVS) guidelines recommend use of non-invasive vascular lab evaluation after revascularization for chronic limb threatening ischemia (CLTI). However, the value of toe arm index (TAI) as an indicator of wound healing in patients with CLTI has not been established. A retrospective review was performed of vascular patients with lower extremity wounds that underwent peripheral vascular intervention. Data regarding patient demographics, comorbidities, TAI, and SVS WIfI score were collected. A total of 173 patients (67.7 ± 10.9 years; 71.1% male) were identified with lower extremity wounds. Mean preoperative TAI was 0.21 ± 0.16 and mean SVS WIfI score was 3.02 ± 1.08. The mean postoperative TAI was 0.36 ± 0.20. Sixty-six percent of patients (97) healed within 1 year of revascularization. Patients that healed within 1 year without major amputation had a higher postoperative TAI (0.38 versus 0.30, p=.03). This was similar in the subset of patients with diabetes (0.37 in healed versus 0.28 in non-healed, p=.01). A Youden index identified a TAI value of 0.30 that optimized sensitivity and specificity for wound healing. The TAI value of 0.30 may be a clinically important threshold to identify wound healing potential in patients with CLTI. UC Davis 34 th Annual Undergraduate Research, Scholarship and Creative Activities Conference 114 Peer Support and Stress in School-Aged Children: Associations With Hair Cortisol Camille Lussier
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UC Davis / MED: Surgery / 2023
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Brian Luong