Authors:

Sanjana Muniraj

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About Paper:

Atopic Dermatitis (AD) is a chronic inflammatory skin disorder with millions of cases annually in the US. The standard treatment to control AD is the topical application of corticosteroids, which may result in dermal atrophy. In this study, a cell penetrating MK2- inhibitor peptide YARA (YARAAARQARAKALNRQGLVAA) was encapsulated into thermo-responsive pNIPAM nanoparticles (NP), which were incorporated into chitosan hydrogels to promote local drug delivery, improve moisture and anti-inflammatory activity, while reducing adverse effects. The NPs showed a mean diameter of 361.5 ± 4.6nm, negative ζ potential (-28.3mV), high encapsulation efficiency (>50%) and did not show cytotoxic effects in human fibroblasts and keratinocytes, suggesting its safety for topical applications. Both nanoparticles and hydrogels were able to improve the release kinetics of YARA up to 120h and deliver 2 and 4-fold more YARA into viable skin layers of porcine skin in vitro at 12h post-application than the nonencapsulated compound in intact and impaired barrier conditions. Furthermore, the NPs treatment decreased the levels of IL-1β, TNF-α, INF-α and IL-10 cytokines up to 9-fold compared with the non-treated group of human keratinocytes under inflammatory conditions. These results suggest that YARA loaded nanoparticles incorporated into chitosan hydrogel is a promising treatment for AD. The Influence of Parent Pitch on Infant Vocabulary Size Jasmine Munoz

Source:

UC Davis / Biomedical Engineering / 2023

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Co-authors:

Sanjana Muniraj