Authors:

Ilara Yilmaz

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About Paper:

The specialized cell division of meiosis is required to form haploid gametes from a diploid germ cell. One key factor in proper chromosomal segregation in meiosis is the formation of the synaptonemal complex (SC), a protein-packed structure between chromosome pairs. The SC is composed of an axis along each chromosome, transverse filaments connecting the axes and a central element stabilizing the transverse filaments. Work from mice shows a component of the central element, SYCE2, is important to extend the SC after it initiates at the sites of meiotically induced DNA double strand breaks (DSBs). DSBs are important for the exchange of genetic material, a process also assisted by the SC. To determine if full-length chromosome alignment is mediated by SC extension, we used CRISPR-Cas9 to create a syce2 knockout strain to prevent the SC from fully forming. Using immunofluorescence microscopy to probe chromosome-associated proteins, we rarely observed full-length chromosome alignment in syce2 mutants and DSBs are less efficiently repaired. Surprisingly, our preliminary data indicates that syce2 mutants still produce healthy offspring. These results inform our understanding of genetic factors affecting chromosome missegregation, miscarriages and infertility. Vitamin A Deficiency: An Artistic Overview of Pathology and Cultural and Biomedical Interventions Claire Young

Source:

UC Davis / Molecular & Cellular Bio / 2023

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Co-authors:

Ilara Yilmaz