Authors:

Adam Bynder

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About Paper:

Ovarian reserve determines female reproductive lifespan and is defined by the quantity and viability of dormant oocytes. Several events are essential for the maintenance of the oocyte reserve, such as the nuclear localization of FOXO3. Before follicle activation, oocytes will stay dormant in the primordial follicle stage. However, it is unclear how the chromatin state of the oocyte is regulated for that long term dormancy. We hypothesize that the repressive chromatin remodeler CHD4 establishes a chromatin state that is essential for maintaining ovarian reserve. To validate this hypothesis, Chd4 cKO mice were generated using germ cell-specific Vasa-Cre mice and Chd4 flox mice. We used immunostaining followed by oocyte counting to confirm this knockout. Our immunostaining data showed that the CHD4 protein is absent from P1 ovaries in Chd4-KO mice. In addition, there was a significant decrease in both the number of oocytes and the number of oocytes with nuclear localization of FOXO3 in these Chd4-KO ovaries. Further research could focus on this phenomenon in order to discover more about dormancy in oocytes. We conclude that CHD4 is essential for the ovarian reserve formation, including the nuclear localization of FOXO3.  Methods for Analysis of MRI and PET Images for Monkey Model of Social Connectedness Anelise Caceres

Source:

UC Davis / Microbiology & Molec Genetics / 2024

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Co-authors:

Adam Bynder