Aditi
Thambala
Sponsor: Yayoi Takamura, Ph.D. Materials Science&engineering For next-generation computing and memory devices, increasing computational speed and enhancing data retention and energy efficiency are essential. The patterning of magnetic thin films into micromagnets of varying geometry (i.e., size, shape, and orientation) is an essential aspect of their incorporation into these types of devices as each micromagnet's unique spin textures are governed by factors such as its magnetocrystalline and shape anisotropies . In this study, micromagnets were patterned into a ferromagnetic complex oxide thin film with the composition La0.7Sr0.3MnO3. Photoemission electron microscopy was used to image the distinct spin textures that resulted and to determine their dependence on micromagnet geometry. An image analysis process was developed to automate the identification and characterization of the observed spin textures, enabling precise statistical analysis and deep insights into their energetic behavior. This work will enhance our understanding of magnetic spin textures in micromagnets and establish the foundation for integrating these structures into next-generation devices. Can RNase H2 Overexpression Suppress DNA Damage Induced upon RNA Splicing Dysfunction
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Aditi Thambala
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R-loops are RNA:DNA hybrid structures that can form during transcription. Research increasingly suggests that under pathological conditions, such as RNA splicing inhibition, harmful R-loop formation can result in DNA damage and genomic instability. Genomic instability is a hallmark of human cancers. RNase H2 is a specialized enzyme equipped with the biochemical activity to degrade RNA strands in RNA:DNA hybrids. My hypothesis is that RNase H2 overexpression will lead to a decrease in the amount of DNA damage under pathological conditions, through the resolution of R-loops. To test this hypothesis, I will generate an inducible RNase H2 expression construct and introduce it into human HCT116 cells genetically engineered to allow depletion of the U2-associated Aquarius (AQR) splicing factor. AQR depletion increases DNA damage as measured by immunofluorescence microscopy against DNA damage markers. I will test whether induction of RNase H2 expression under conditions of AQR depletion can rescue the DNA damage caused by impaired splicing. If so, this result will implicate harmful R-loops as a potential source of the DNA damage phenotypes. In the future, we hope to improve our understanding of RNase H2 function and R-loop associated DNA damage, as well as create new therapeutics for cancer treatments. UC Davis 36 th Annual Undergraduate Research, Scholarship and Creative Activities Conference 274 Understanding the Current Landscape of Artificial Intelligence and Machine Learning Modeling and Techniques for Preeclampsia Prediction and Other Cardiovascular Outcomes. Shanthoshi Thamilseran
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UC Davis / Molecular & Cellular Bio / 2025
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Aditi Thambala