Catherine
Pham
Sponsor: Gabriela Grigorean, Ph.D. UC Davis Genome Center Plasma is an attractive source of protein biomarkers for unlimited diseases, yet proteomic analysis is challenged by a high dynamic range and dominance of abundant proteins that suppress detection of low-abundance species. I present a bead-enrichment technique, using Nanotrap PEAK magnetic beads, that greatly reduces the overabundant proteins such as albumin and antibodies, thus enhancing the low abundance part of the bio- fluid proteome. This underscores the need for robust and reproducible enrichment workflows suitable for clinical and core facility operations. I analyzed human plasma. To compare methods, we used the routine digestion devices the Core uses for its digestions, ProtiFi's suspension-trap (S-Trap) processing, to compare with the Nanotrap PEAK beads technique. Nanotrap PEAK digestion substantially increased detection of proteins of biological interest compared to S-Trap digestion. The S-Trap yielded the typical number of IDs of ~600 proteins. The Nanotrap PEAK processing increased this by 2-3 times. We observed functional proteins like cytokines, chemokines, complement and immune factors, hormones, receptors, and antagonists. By improving the visibility of rare proteins, these beads are highly effective for not just core facilities focused on translational biomarker discovery, but any lab interested in human plasma analysis. SOX10 Controls Cadherin Dynamics and Neural Crest Migration in Avian Embryos
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Authors:
Catherine Pham
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Neural crest (NC) cells are a vertebrate-specific population of multipotent embryonic stem cells that give rise to over 30 adult cell types, including craniofacial cartilage, bone and peripheral nervous system. During development, NC cells undergo epithelial-to-mesenchymal transition (EMT), altering cell-cell adhesion to delaminate from the neural tube and migrate throughout the embryo. Processes are regulated by neural crest gene regulatory network (GRN). Dynamic regulation of cadherin- mediated adhesion is essential for NC EMT and migration. Neural cadherin (NCAD) is downregulated to permit delamination, while epithelial cadherin (ECAD) is maintained in early migratory NC cells. SOX10- a key transcription factor within the NC GRN, is required for NC migration, yet its interactions with cadherins remain poorly understood. We observed that SOX10 overexpression causes premature differentiation and arrested NC migration in chicken embryos, while promoting premature NC migration in quail embryos. We hypothesized that species-specific phenotypes arise from differential regulation of cadherin expression. We performed ex ovo injections of a SOX10-GFP expression plasmid into chicken and quail embryos at gastrulation stage and analyzed NCAD and ECAD protein expression during NC migration using immunohistochemistry. Results demonstrate that SOX10 overexpression drives divergent cadherin expression patterns across species, highlighting a conserved factor with species-specific regulatory outcomes. Evaluating Vignette Usage in Moral Judgment Research: A Scoping Review Dena Pham
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UC Davis / VM: Anat Physio & Cell Biology / 2026
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Catherine Pham