Katherine
Wheeler
Sponsor: Cheemeng Tan, Ph.D. Biomedical Engineering Extracellular vesicles (EVs) are lipid composed nanoparticles released from all cell types in the body. EVs are decorated with a multitude of different surface proteins, which enable them to exhibit therapeutic efficacy. These different surface proteins, however, lead to heterogenous vesicle populations which makes linking protein presentation to function a difficult task. In our previous work, we utilized cell-free protein synthesis (CFPS) to modulate liposomes to incorporate the desired surface protein in a bottom-up, single protein display platform. A CFPS platform where multiple different proteins are integrated on a liposome would greatly enhance our understanding of protein interactions and improve efficiency of therapeutic outcomes, such as wound healing and neuroprotection. In this bottom-up combinatorial study, we tested three different methods of demonstrating dual expression of CD99 and MMP14 proteins, one of which included filtration. Our results show that generating one protein using cell- free protein synthesis, filtering and reconcentrating these vesicles and utilizing them in a second cell-free reaction demonstrates the highest rate of dual-protein display. With this demonstration of the incorporation of multiple proteins, the CFPS platform can dramatically advance studies to better understand the inherent heterogeneity in EVs, ultimately resulting in more successful therapeutic outcomes. Can Friendship Network Diversity Compensate for Less Frequent Socializing? Implications for Emerging Adults' Perceptions of Social Support
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Authors:
Katherine Wheeler
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Quality time spent with friends, coworkers, and peers helps emerging adults meet emotional and belonging needs; however, socializing frequency alone may not be sufficient. Social networks comprised of diverse others (e.g., different racial backgrounds) across different contexts (school, work, or other) may address gaps by providing a wider breadth of resources and experiences to derive support from. I hypothesized that individuals who (1) socialize more frequently and (2) have more diverse networks would perceive greater support, and that (3) network diversity would moderate this relationship such that individuals with diverse networks would perceive greater support, even when socializing less. Regressions with time spent socializing (α = .93) and network diversity in school (α = .85), work (α = .88), or outside contexts (α = .89) predicting perceived social support (α = .78) were run with a diverse sample of college-aged emerging adults in California and Oregon (N = 277; 59% female). Results indicated that frequent socializing was associated with greater perceived support (p < .001); however, network diversity across contexts was not, nor did it moderate the relationship (ps > .05). Additional analyses will examine possible interaction effects of gender and ethnicity to inform support interventions during emerging adulthood. Exploring Transcriptional Regulation of T6SS in Plant Pathogenic Ralstonia by Reanalyzing Public Transcriptome Data Valerie Whitfield
Source:
UC Davis / Human Ecology / 2026
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Co-authors:
Katherine Wheeler