Authors:

Cameron Azizi

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About Paper:

Metabolism Osteoarthritis (OA) is a degenerative joint disease characterized by cartilage breakdown and chronic inflammation following injury. While inflammation is essential for tissue repair, failure of resolution leads to persistent inflammation and disease progression. Current treatments primarily address symptoms, highlighting the need for disease-modifying therapies. This project investigates the therapeutic potential 84 UNIVERSITY OF OREGON • 2026 UNDERGRADUATE RESEARCH SYMPOSIUM TABLE OF CONTENTS of Specialized Pro-resolving Lipid Mediators (SPMs), derived from docosahexaenoic acid (DHA), in promoting inflammatory resolution and regenerative signaling in chondrocytes. Human and bovine articular chondrocytes were cultured and stimulated with IL-1β to model an OA-like inflammatory environment. Cells were then treated with DHA, Resolvin D1 (RvD1), or a synthetic analogue (WKYMVm). Metabolic activity was assessed using the Alamar Blue assay. Results demonstrate a dose-dependent response to lipid mediators. Low DHA concentrations (5-20 μM) increased metabolic activity, while concentrations ≥40 μM reduced activity, indicating cytotoxic effects. RvD1 showed no significant effect, and synthetic analogues exhibited cytotoxicity at higher doses. This work highlights the importance of dosing in SPM-based therapies and supports further investigation into their regenerative potential in OA.

Source:

University of Oregon / 2026

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Co-authors:

Cameron Azizi