Authors:

Amandeep Kaur LNU

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In line with global public health needs, there is an urgent need to discover new bioactive compounds that can be developed into antibiotics against antimicrobial-resistant pathogens. Mycobacterium tuberculosis, non-tuberculous mycobacteria (NTM; such as Mycobacterium abscessus, Mycobacterium avium, and Mycobacterium ulcerans), and ESKAPE pathogens are clinically important organisms that are notorious for their multidrug resistance. Streptomycetes are known to synthesize a vast array of secondary metabolites and are therefore a valuable source of natural products with diverse biological activities. This study focuses on soil-derived Actinobacteria as a source of compounds for antimicrobial drug discovery. Soil samples were collected from several locations around Chicago. Using sterile water, serial dilutions were prepared and plated on three different selective media for the isolation of Actinobacteria. Colonies appeared after incubation for 3 to 7 days and were streaked onto the corresponding agar plates to obtain pure cultures. Selected isolates were then fermented in various media for 7 days. After fermentation, extracts were prepared and subjected to preliminary biological screening against M. tuberculosis, NTM, and ESKAPE pathogens. A total of 39 isolates showed activity against one or more of the target pathogens. Isolates 2AD1, 3BC6, 2AD4, 4AR7, 1AK3, and 3BC9 showed notable activity against Staphylococcus aureus, as well as against M. tuberculosis, M. ulcerans, and M. abscessus. Activity was also observed against Borrelia burgdorferi, the causative agent of Lyme disease. We are now working on identifying these isolates and purifying the active compounds for structure elucidation and determination of minimum inhibitory concentrations (MICs). SCOP SHT OHSS HOC OHHH COOH OCSOS COSCO HO OCHS SOO SHO OCS HOG OOCOOOO

Source:

University of Illinois Chicago

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Co-authors:

Amandeep Kaur LNU