Authors:

Veera Byrne

Date Created:

Not specified

Course Title:

Professor:

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About Paper:

Antibiotics are widely used in clinical settings to combat bacterial infections. When the antibiotics are removed from the environment, the resistant bacterial populations may experience a cost to fitness, resulting in a lower fitness than those evolved without antibiotics. We posed the following question: Do E. coli populations resistant to tobramycin pay a cost to fitness when antibiotic is removed from the environment? We evolved bacterial populations for 24 days under tobramycin selection in either nitrogen-limited or carbon-limited conditions. We then tested whether the evolved Escherichia coli lost fitness in their corresponding nutrient-limited environment in the absence of antibiotic. The evolved populations competed head-to-head against the ancestor for the limiting nutrient. The magnitude of fitness loss did not differ between carbon-limited and nitrogen-limited environments. Fitness of resistant populations varied, with some, but not all, showing a cost to resistance. This suggests that some resistant populations may persist in the absence of antibiotics, while others would be outcompeted by antibiotic-sensitive strains. OPC SHMOOSSHOOEO SO OCH ECHO EO HOSS OL OOOO OOCOOHOSCOOCOOOOO GGDDHOHHHDDDDDIDDDIDHDIIDDDDDHDDIDIIIDDDDIIIIISD

Source:

Loyola University Chicago

Topics:

No topics listed

Co-authors:

Veera Byrne