Carly
Mccurry
Sponsor: Richard Michelmore, Ph.D. MED: Medical Microbiology & Imm Reporter genes are an important tool for molecular biology research, allowing researchers to understand when and where genes are being expressed in organisms. GFP is the most well- known and used reporter gene, which fluoresces green under UV light. Fluorescent and non-fluorescent proteins that come in all colors of the rainbow were generated for bacterial expression by biologists, and some of them can be viewed with the naked eye under ambient light (chromoproteins). To aid research on promoters for transgene expression in lettuce, I used Golden Gate cloning to make plant expression constructs for the rainbow of chromoprotein genes and assessed their use as reporter genes in lettuce. I made constructs using the LjUBI promoter for all six chromoprotein constructs and also made red, blue, and purple chromoprotein constructs using the LsUBI promoter. In transient assays, the red, orange, yellow, and green chromoproteins displayed fluorescent expression in lettuce with the purple-red reporter RUBY being used as a control due to its high visibility in lettuce and transient assays. Non-fluorescent blue and purple chromoproteins were visible to the naked eye in certain tissues of stably transgenic lettuce, with the LjUBI promoter showing greater expression than the LsUBI promoter. Investigating the Role of Amyloid Beta Agonism of Beta-2 Adrenergic Receptors on Amyloid Precursor Protein Processing in the Brain
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Authors:
Carly Mccurry
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Adrenergic neurons, originating from the locus coeruleus, are the first neurons to show neurodegeneration in Alzheimer's Disease (AD). Norepinephrine binds to a variety of postsynaptic receptors, including beta-2 adrenergic receptors (β2AR). B2AR is highly expressed in the brain and can directly interact with soluble Aβ, a main player in AD development. Previous work from the lab found that Aβ binding to β2AR, followed by GRK phosphorylation, can trigger endocytosis and receptor degradation, causing a net decrease in β2AR in neuron membranes. Additionally, studies have shown that β2AR internalization can facilitate endocytosis of two transmembrane proteins involved in Aβ production: amyloid precursor protein (APP) and γ-secretase. When internalized, the acidic environment facilitates γ-secretase activation in an endosome, which increases APP processing and Aβ production. We propose a positive feedback mechanism whereby β2AR degradation, via Aβ binding, promotes APP cleavage and further Aβ production. This leads to further accumulation of Aβ and pathogenesis of AD. To test our hypothesis, we used a genetic approach to interfere with this pathway in a mouse model of AD and quantified proteins of interest. By disrupting this cycle, we expect a decrease in Aβ accumulation, neuron loss, neuroinflammation, and alleviation of symptoms in AD model mice. From Authenticity to Appropriation: Examining the Impact of AAVE Misappropriation on Professional Spheres Nadia McGhee
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UC Davis / MED: Pharmacology / 2024
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Carly Mccurry