Tatiana
Pechnikova
Sponsor: Barbara Shacklett, Ph.D. MED: Int Med Infectious Dis HIV is a chronic viral infection that has taken the lives of an estimated 40 million people worldwide to date. HIV infection is fatal if untreated; however, most people with HIV develop strong immune responses, including both T-cells and B-cells/antibodies, that can be measured and characterized in the laboratory. A better understanding of these immune responses may help the research community to develop successful vaccines and/or immunotherapies for HIV. We routinely use Flow Cytometry to characterize immune cells in blood and tissue samples from people living with HIV. We are currently developing a 17-color "spectral" flow cytometry panel to assess immune cell subsets. This process involves testing new antibody-fluorochrome combinations to identify those that provide the clearest depiction of the cell populations of interest, using positive and negative control samples. Once the panel is fully optimized, we will use it to characterize CD4 and CD8 T-cells, B-cells, macrophages, NK cells, MAIT cells, Regulatory T-cells, memory/effector T-cell subsets, and markers for T-cell activation and senescence. These studies are directed towards better understanding the roles played by individual immune cell subsets in fighting HIV infection. Uncovering the role of OLFML3-induced malignancy in human GBM cells
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Tatiana Pechnikova
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Glioblastoma (GBM) is an aggressive, uniformly fatal primary brain tumor in adults. There has not been a new treatment approved by the FDA since 2009, highlighting the urgent need for a new therapeutic target. Our laboratory has identified that olfactomedin-like 3 (OLFML3), a secreted glycoprotein, has multiple pro-tumorigenic roles in GBM. My preliminary data has demonstrated that exposure to recombinant human OLFML3 (rhOLFML3) enhances features of malignancy in human GBM cells through increased cellular migration. To determine the OLFML3- responsive pathways, we have evaluated the transcriptome of human GBM cells following exposure to rhOLFML3 via RNA sequencing. Treatment with rhOLFML3 induced up-regulation of several genes in human GBM cell lines. Genes with the greatest up-regulation, interleukin 6 (IL6) and C-X-C motif chemokine ligand 10 (CXCL10), are also critical drivers of GBM progression. I am currently evaluating additional patient-derived GBM cell lines with variable molecular profiles (e.g., EGFR amplification, MGMT methylation) for phenotypic responses to rhOLFML3 and will examine OLFML3-responsive pathways in each cell line at the protein level via western blot. This study will advance our understanding of OLFML3 singling in human glioma cells and inform the development of OLFML3-targeted therapeutic strategies. Natural Sequence Diversity of Maize Diterpene Synthase, ZmKSL1, Influences Diterpenoid Production in Nicotiana benthamiana Jedidiah Peek
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UC Davis / VM: Surg/Rad Science / 2024
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Tatiana Pechnikova