Daniela
Perla
Sponsor: Susan Lott, Ph.D. Evolution & Ecology Genome editing is a developing field that has opened the possibility of many scientific advancements from agriculture to human health improvement. Concerns have arisen about the boundaries and unintended consequences of gene editing in humans as its technologies and applications continue to evolve. Somatic gene editing clinical trials for various diseases have begun to be applied, but germline gene editing remains widely restricted. This research explores the moral and ethical implications of modern genetic technologies, delving into the development of gene editing, its societal impact, and future applications in humans. A comprehensive literature review provides insight into the current discourse of gene editing technologies. Surveys and interviews targeting undergraduates majoring in Genetics and biological sciences, graduate students in the Integrative Genetics and Genomics program, and professionals actively engaged in the genetics field provide a qualitative exploration of their perspectives and opinions concerning the future of gene editing. These findings seek to inform discussions and decision-making processes related to the ethical use and regulation of gene editing in the human germline, ultimately contributing to the responsible advancement of genetic research applications. Validation of CHD8 Protein-Protein Interactions with PAF Complex in Cultured Cell Lines
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Authors:
Daniela Perla
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CHD8 haploinsufficiency has been linked to autism-like phenotypes. However, its interactions with other proteins in the nucleus of cells remains unknown. After validation of candidate protein targets via mass spectrometry, a Proximity Ligation Assay was carried out. This technique allows for the in-situ validation of protein-protein interactions on cultured cell lines. Probes are bound to constant regions of primary antibodies and the resulting signal is amplified via DNA hybridization and polymerization. Our results have allowed for the validation of protein-protein interactions between CHD8 and the PAF complex in Hek Cells. The PAF complex is a 5 sub-unit protein complex known to regulate chromatin modifications, gene transcription, and RNA polymerase II (PolII) elongation. An immunocytochemistry procedure was also achieved on Hek Cells to map the expression of CHD8 and candidate PAF proteins. We are currently working on analyzing these interactions in neurons, and are also exploring protein-protein interactions between CHD8 and RBM8A. A storybook intervention study on the development of uncertainty monitoring 3- and 4-year-old children Chiara Perni
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UC Davis / Neuro Physio & Behavior / 2024
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Daniela Perla