Authors:

Shruti Nene

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Gene regulation is critical in male germ cell differentiation to produce functional sperm. The poly ADP-ribose polymerase (PARP) family of proteins has been implicated in male meiosis, specifically in the DNA damage response (DDR) pathway that functions in meiotic sex chromosome inactivation (MSCI). In this study, we aim to understand the role of the PARP family in male meiosis. To this end, we used an ex vivo male germ cell culture model treated with the PARP inhibitor Olaparib and examined its effects on gene expression in spermatogenesis. Preliminary results identify over a thousand differentially expressed genes in treated cells. These genes have a variety of functions, including oxidative stress response, DNA damage response, and other gene regulatory processes of meiotic spermatocytes and post- meiotic spermatids. However, the results do not necessarily indicate that MSCI is affected. During male germ cell differentiation, postmeiotic round spermatids seem to be most affected by this inhibition. Our study cautions that male infertility should be evaluated when the PARP inhibitor Olaparib is used in patients. Voices of Migration: A Narrative on Immigration, Labor, and Bicultural Identity Emily Neri

Source:

UC Davis / Microbiology & Molec Genetics / 2025

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Co-authors:

Shruti Nene