Authors:

Wendy Tran

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About Paper:

Lipid nanoparticles (LNPs) have been widely used to introduce RNA into cells, a vital process in the use of therapeutics to treat various diseases. However, some current commercially available compounds used to introduce RNA into cells have low efficiency for certain cell lines. This research project aims to look at the effect that introduction of silyl groups have on transfection efficiency and RNA encapsulation through synthesizing novel, ionizable silyl LNPs. Previous work done on LNPs made from cationic lipids demonstrated that three of the synthesized lipids had increased stability and transfection efficiency due to the addition of the silyl group into the cationic lipids. From this work, we hypothesize that the incorporation of silyl groups into ionizable lipids will increase LNP stability while increasing transfection efficiency of RNA. Hydrosilylation, the addition of Si-H bonds to unsaturated bonds, offers advantages of accessibility to many novel silyl lipid structures and will be used to synthesize the LNPs. To measure RNA and DNA encapsulation efficiency, a fluorescence assay was done on the silyl LNPs made from the ionizable lipids and cationic lipids. Future work includes evaluation of transfection efficiency of LNPs with the varying ratios of cationic and ionizable silyl lipids. Riluzole as a Potential Treatment for Acute Organophosphate Intoxication Izzy Triana

Source:

UC Davis / Chemistry / 2025

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Co-authors:

Wendy Tran