Authors:

Skyler Campbell

Date Created:

Not specified

Course Title:

Professor:

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About Paper:

We sought to develop a point-of-care (POC) diagnostic device for pathogen detection within 60 minutes in a non-laboratory setting. In recent years, researchers have utilized DNA amplification techniques that leverage color-based indicators to quickly identify pathogens from just a few copies of genetic material. These tests allow an easy visual way to read results; if the color of the sample changes, the pathogen is present. However, if a user wants to know the precise quantity of pathogen present, image analysis must be conducted on the samples. We aimed to conduct this thorough quantitative analysis within the POC setting and have successfully developed a device that consolidates the entire detection process into a portable, user-friendly package. The device's components cover the two key parts of achieving data-informed results: collection and analysis. The samples are placed in the device and are heated up to 65°C, which is the temperature needed for the amplification reaction. A camera captures time-lapse images to track color change as the reaction progresses. A Raspberry Pi single-board computer contained in the device then allows it to automatically run image processing code on the collected snapshots. We aim to test the device for proof-of-concept such that an untrained user can receive automated quantitative results from our device within 60 minutes. We would like to continue developing the device for application across different point-of-care settings. The device offers an appealing opportunity to have both precision and user-friendliness in portable tests, without having to sacrifice either benefit.

Source:

Purdue University / 2023

Topics:

No topics listed

Co-authors:

Skyler Campbell