Authors:

Tierah Macon

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About Paper:

Desorption electrospray ionization (DESI) is an ambient ionization method in which charged microdroplets, carried by a sheath gas, impact on a sample's surface thereby generating secondary splashed droplets that contain the ionized analyte. In this case, the ionized analytes were characterized using two-dimensional tandem mass spectrometry (2D-MS/MS)-an untargeted method of analyzing complex mixtures-featuring one dimension of ion abundance and two dimensions of mass-to-charge: precursor ion m/z and their resulting product ion m/z values. When these two methods are coupled, high throughput experimentation (HTE) with both broad chemical structural coverage (precursor ion and product ion ranges (m/z) 400 - 1100 and 150 - 800, respectively, per second) and unprecedented speed (3 seconds per sample for a 3-scan average) is possible. In this work, two biological systems-bacteria and banked mouse brain tissue lysates-were analyzed using DESI 2D-MS/MS to demonstrate how these methods are applicable to various biological systems with minimal sample preparation and can be used with HTE. Bacteria were cultured in the wells of a polypropylene 96 well plate with an equal amount of lysis solvent added to each well, while a premade solution of lysates from the banked brain tissue was used. The lipid profiles of each of the systems were analyzed using DESI 2D-MS/MS after spotting 2-3 µL of the lysates on a Teflon slide. Our results show that characteristic lipid profiles and structural information from precursor/product ions of each of the systems can be rapidly obtained by HTE with minimal sample preparation.

Source:

Purdue University / 2023

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Co-authors:

Tierah Macon