Authors:

Aidan Edge

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About Paper:

Volatile organic compounds (VOCs) are low molecular weight lipophilic molecules that are easily vaporized off the surface of plants and released into the atmosphere. VOCs play various roles in plant-plant interactions, pollinator attraction, allelopathy, and immune responses against pathogens and herbivores. Until recently, it was assumed that VOCs simply diffuse across the cell membrane onto the surface of the plant. For it to be possible to achieve this emission rate via simple diffusion, predictive modeling has theorized that the concentration of VOCs needed in the plasma membrane would result in cell toxicity. Attention has turned to uncovering the method of volatile transport through the cell onto the cell surface. RNA-seq data has shown that Petunia hybrida have three genes that fall into the target expression profile for high emission times of VOCs: PhSV2-1, PhSV2-2, PhSV2-3. These three genes are homologs of mouse synaptic vesicle protein 2A (MmSV2A); a protein known for its role in vesicle docking on target membranes. Generation of stable PhSV2 knockdown lines and analysis of VOC emissions revealed that emissions decrease in the PhSV2 knockdown compared to the wild type. This investigation aims to elucidate the mechanism behind the relationship of PhSV2s and VOC emission. We hypothesize that silencing PhSV2 will prevent vesicle trafficking of PhABCG1 (an identified VOC transporter) to the plasma membrane, and ultimately, reduce the level of VOC emission. This will be approached by visualizing the differences in the presence of PhABCG1 on the plasma membrane in PhSV2 knockdown and wild type petunias using Western blotting.

Source:

Purdue University / 2023

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Co-authors:

Aidan Edge