Authors:

Marcella Dibble

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About Paper:

Eisosomes are protein complexes that assemble at the plasma membrane in fungi and are important for metabolic homeostasis, nutrient uptake, and regulating stress response signaling pathways, including the control of sphingolipid metabolism. Sphingolipids are critical signaling molecules and structural components of the cell membrane. The Hall lab recently discovered that the protein phosphatase PP2ARts1 controls the localization of eisosomes at the plasma membrane. The biological significance of eisosome regulation by PP2ARts1 is unclear. Since PP2ARts1 and eisosomes both have been linked to sphingolipid metabolism independently, we hypothesized that PP2ARts1 regulation of eisosome localization might play a role in sphingolipid homeostasis. To test this hypothesis, I used agar plate spotting assays to measure the sensitivity of yeast cells lacking the Rts1 subunit of PP2ARts1 to the sphingolipid synthesis inhibitor myriocin. I used the auxin-inducible degron (AID) system and gene deletions to disrupt Rts1, and plasmids containing the RTS1 gene to complement Δrts1. My results from the Δrts1 complementation assays and the AID system suggest that previous conclusions on the role of PP2ARts1 in sphingolipid metabolism need to be revised. We are currently exploring other hypotheses for the biological significance of eisosome regulation by PP2ARts1.

Source:

Purdue University / 2023

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Co-authors:

Marcella Dibble