Authors:

Karla Alvarado

Date Created:

Not specified

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Professor:

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About Paper:

In the medical field, biomedical ultrasound imaging has become an essential technique in managing diseases and improving diagnoses. Ultrasound contrast agents are small spheres of gas with low solubility in blood. However, ultrasound imaging has limitations like distinguishing tissue features which led to the formulation of nanobubbles as ultrasound contrast agents, which led to the discussion of which is the best composition that will provide uniform size, stability, and echogenicity. This study aims to find how the lipid composition affects the nanobubble's size, stability, and echogenicity. To create the lipid layer, DPPA, DPPE, and DPPC were the lipids of choice due to their ability to develop sphere-shaped particles and biocompatibility with living organisms. We used a 1:2:8 ratio to mix lipids with 1 mg of DiI, a fluorescent compound and later mixed them with chloroform. After combining the lipids, the DiI, and the chloroform in a 2 mL vial, it heated, which allowed the lipid shell to form. After removing contaminants, we rehydrated the lipid shell with PBS, a buffer, and entrapped the gas of choice in the vial. To measure, we use a technique called Dynamic Light Scattering, which allows us to measure the uniformity in size of the nanoparticles. Ultimately, we want to obtain data that will provide differences in size, behavior, echogenicity, and stability.

Source:

Purdue University / 2023

Topics:

No topics listed

Co-authors:

Karla Alvarado