Authors:

Elizabeth Hern

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About Paper:

Agrobacterium tumefaciens is a soil bacterium capable of infecting cells by transferring and integrating its DNA (T-DNA) into the plant genome. This DNA transfer process has sparked interest in its use in plant biotechnology. Agrobacterium also transfers virulence proteins, including VirE2, which facilitates T-DNA transfer to the plant nucleus. This study analyzes the intracellular trafficking of VirE2 protein and evaluates its interaction with PDLP5 (Plasmodesmata located protein 5) and SYTA1 (Synaptotagmin 1) proteins. PDLP5 is essential for cell-cell communication and SYTA1 regulates protein transport at the plasma membrane. The interactions of VirE2 with plant proteins during VirE2 intracellular trafficking are poorly understood despite its importance in Agrobacterium infection. Clarifying these interactions will inform us about how infections work and how hosts and pathogens interact. Fluorescence techniques including a split GFP (Green Fluorescent Protein) system and BiFC (Bimolecular Fluorescence Complementation) will be used to monitor VirE2 during Agrobacterium infection and investigate protein interactions. The GFP system will allow us to determine what happens to VirE2 within the plant cell upon T-DNA transfer. BiFC will allow the detection of fluorescent signals that examine the interaction of VirE2 protein with PDLP5 and SYTA1. This study will provide more information about the infection process of Agrobacterium tumefaciens and the behavior of VirE2, one of its most important virulence proteins.

Source:

Purdue University / 2023

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Co-authors:

Elizabeth Hern