Authors:

Karlianie Rivera

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About Paper:

Calcium is a key participant in a variety of signaling pathways that preserve homeostasis after injury. Previous research has demonstrated that extracellular calcium is responsible for tissue contraction following tissue damage, implicating the calcium sensing receptor (CaSR), which senses extracellular calcium levels, as a prime research target. Given that there is limited knowledge of the involvement of CaSR in wound healing, the objective of our study is to characterize its role during the embryonic zebrafish wound response. Based on results with chelators, extracellular calcium is required for wound contraction. Furthermore, treatment with NPS-2143, a CaSR inhibitor, causes sustained contraction and tissue death. To verify these results, embryos homozygous for a CaSR knockout were wounded and observed using light microscopy. Preliminary results show that the knockout demonstrates the same phenotypic results as treatment with a CaSR inhibitor, most notably sustained contraction during the wound response. Further genotyping will confirm that the modified phenotype truly results from the CaSR mutation. Ultimately, studying the potential role of the molecules involved in calcium signaling pathways in response to injury will provide insight into the multi-level tissue scale phenomenon of wound response.

Source:

Purdue University / 2023

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Co-authors:

Karlianie Rivera