Authors:

Cristian D. Rosario-Marcano

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About Paper:

Plants have developed defense mechanisms to fight against potential pathogens, mainly involving cytoskeletal rearrangement. Actin filament nucleators are key players in cytoskeletal array construction, as nucleators initiate the formation of new actin filaments. One of these nucleators is the Actin-Related Protein 2/3 complex (Arp 2/3). Studies have shown the Arp2/3 to be one of the nucleators responsible for penetration resistance regulation to powdery mildew since actin patch-like structures have been observed at fungal penetration sites, but the penetration mechanism of powdery mildew is mainly mechanical. If Arp 2/3 is a nucleator responsible for nucleation in response to chemical stimuli remains to be observed. To test this, five-day old Arabidopsis thaliana hypocotyl cells were treated with a MAMP to induce a chemical stimuli and images were acquired on a TIRF microscope to observe single filament growth events. Three types of growth events were described: side branches, de novo and end growths. ImageJ software was utilized for filament analysis on each acquired image, and single filament measurements taken on each one, including filament lifetime, elongation and severing rate. The results of this experiment would help determine Arp2/3 complex's role in a plant cell's response to pathogen invasion and help identify other potential molecular players in plant's immune response.

Source:

Purdue University / 2023

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Co-authors:

Cristian D. Rosario-Marcano