Authors:

Faith Scott

Date Created:

Not specified

Course Title:

Professor:

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About Paper:

The risk of many chronic diseases increases with age. With the rapidly growing elderly population, better understanding the biology of aging is critical. Advances in proteomics now allows for the assessment of thousands of proteins in biological matrices, which can be translated into clinical biomarkers. This project aims to investigate the biology of aging and identify potential biomarkers of aging utilizing proteomics techniques. We will perform both untargeted and targeted proteomic analyses of liver tissues collected from young and old mice to identify specific proteins that are differentially regulated with age. We will first generate a protein library using comparative proteomics to identify differentially regulated liver proteins. We will use this library to develop Multiple-Reaction-Monitoring-Mass Spectrometry (MRM-MS) assays which facilitate the accurate quantification of proteins within different samples. For MRM assays, we will select several previously reported tumor suppressor proteins as well as several newly identified proteins from our study to quantify and assess differences in the specific peptides corresponding to the proteins of interest. MRM-quantification of target peptides allows for the precise determination of which proteins are up- or down-regulated in old mice relative to young counterparts. These protein level changes will highlight which biomarkers are most significant to further explore in later mechanistic studies. Identification of proteins that are differentially regulated during biological aging will facilitate the development of a method to identify age-related biomarkers in a tissue-specific manner. This information is necessary for the future development of therapies to prevent age-associated diseases and promote proper tissue repair in older individuals.

Source:

Purdue University / 2023

Topics:

No topics listed

Co-authors:

Faith Scott