Authors:

Michayla Dinn

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About Paper:

Circadian rhythms are 24-h cycles that regulate physiology and behavior like sleep-wake patterns and cortisol levels and are highly integrated with metabolism. Chronic circadian rhythm disruption, such as shift work, is associated with development of diseases like type II diabetes. The objective was to determine the effect of chronic circadian disruption imposed by light-dark phase shifts (PS) and high fat (HF) diet on diurnal feeding behavior, fecal output, and fecal corticosterone content in ICR mice during pregnancy and lactation. We hypothesized that feed intake, fecal output and fecal corticosterone levels would be altered by HF diet and PS, remaining relatively constant across day and night. A 2X2 factorial study was conducted and virgin mice were placed on control (CON; 10% fat) or high fat (HF; 60% fat) diets and fed ad libitum for 4 weeks. Mice were mated and assigned to light-dark (LD; n=17) or phase shift (PS; n=16). LD were exposed to 12h of light (0600- 1800) and 12h of dark (1800-0600). PS were exposed to a jet-lag paradigm, by shifting the light phase forward 6h every 3d. Mice delivered naturally. Dam and litter were euthanized on lactation d12. Feed intake and fecal output were measured twice daily at 0600 and 1800 to gather day and night data. Corticosterone was extracted from fecal matter and measured using an ELISA. Analysis is ongoing, but preliminary graphs suggest feed intake and fecal output increased from pregnancy to lactation, and early pregnancy showed diurnal differences in both.

Source:

Purdue University / 2023

Topics:

No topics listed

Co-authors:

Michayla Dinn