Authors:

Denisse Victoria Gutierrez

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About Paper:

Human gut microbiota plays a significant role in overall health, impacting disease conditions such as cardiovascular disease, high cholesterol levels and obesity. The gut microbial composition and function can be influenced by factors such as environment, genetics, and diet. Among these factors, diet offers a feasible way to effectively modify the gut microbiota. Dietary fiber, which cannot be absorbed by the human body, serves as a substrate for fermentation by bacteria, resulting in the production of beneficial metabolites. Arabinoxylan (AX) is a common dietary fiber that widely found in cereal crops, which is a complex substrate primarily composed of arabinose and xylose. Recently, AX has emerged as an impactful regulator of gut microflora, which is attributed to its intricate polysaccharide structures, including diverse linkage patterns and branching units. We aimed to analyze the effect of sorghum AX (SAX), a naturally heavily branched AX, on the growth of different human gut isolated microorganisms. Bacteria from phylum Bacteroidetes, Firmicutes, Proteobacteria and Actinobacteria were incubated under anaerobic condition for 24 hours at 37ºC with shaking at 150 RPM. The growth with 0.2% SAX and gut mineral media were evaluated through optical density (OD 600 nm) for 30 hours. The goal is to identify and screen for rapid-growing microorganisms that can thrive on the specific AX substrates, which underlies the fundamental knowledge of how a complex dietary fiber, like AX, governs gut microbial competition. It is expected that SAX can enhance the growth of beneficial strains while suppress the proliferation of pathogenic organisms.

Source:

Purdue University / 2023

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Co-authors:

Denisse Victoria Gutierrez