Authors:

Lorraine Prevost

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About Paper:

C-reactive protein (CRP) is an acute phase protein produced in the liver in response to systemic inflammation. As a more sensitive marker of inflammation in dogs than traditionally used leukograms, CRP is a desirable addition to diagnostic profiles. This study aimed to confirm the validity of the Gentian canine C-reactive protein (cCRP) immunoassay on the PVM Clinical Pathology Lab's Vitros 4600 chemistry analyzer. Serum from 108 dogs was used for a correlation study with the previously validated assay on Cornell University Animal Health Diagnostic Center's Roche Cobas C510 chemistry analyzer. Precision, linearity, and interference studies were also performed. Data obtained from the Vitros 4600 correlated strongly with the Cobas C501 (R2 = 0.9922); however, a negative systemic bias (5.5 mg/dl) was observed and Bland-Altman plots revealed proportional bias. Precision was very good with inter-assay CVs of 2.82 & 2.01 and intra-assay CVs of 2.39 & 3.43 for low and high values, respectively. Linearity was excellent at low and high limits with all R2 >0.998. Interference from lipemia, icterus and hemolysis was negligible at the concentrations tested. Correlation with the previously validated cCRP assay and the observed performance metrics (precision, linearity, interference) support the use of this cCRP assay in the Purdue Clinical Pathology Lab. However, the observed bias on the Vitros 4600 necessitates the establishment of an independent reference interval, currently in development. Once complete, clinicians and researchers can utilize this sensitive diagnostic and monitoring tool for canine inflammatory disease.

Source:

Purdue University / 2023

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Co-authors:

Lorraine Prevost