Joy
Richardson

PURE-PD Characterizing DMP9 Overexpression Lines Regarding Stomata Regulation

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Authors:

Joy Richardson

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DMP 8/9 are proteins expressed at the plasma membrane of sperm cells and are required for double fertilization in Arabidopsis thaliana. In the dmp8/9 mutant, where DMP 8/9 are not expressed, double fertilization cannot occur due to the two sperm cells failing to separate and subsequently fuse with the egg and central cell. To address the molecular function of DMP9, we examined the effects of DMP9 ectopic overexpression in vegetative tissues. Previous studies showed that overexpression lines have reduced plant growth and defects in stomata regulation. To determine how DMP9 interferes with signaling pathways, we examined the effects of known factors involved in stomata regulation including ABA, H2O2, organic acids, and toxic cations in seed germination and root growth. We also examined if the DMP9 ectopic overexpression line presents any stomata developmental defects. Based on the results, we will report a possible model on how overexpression of DMP9 may be interfering with plant osmoregulation.

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Purdue University / 2023

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Co-authors:

Joy Richardson

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