Authors:

Matthew William Margraf

Date Created:

Not specified

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Professor:

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About Paper:

Intrathecal injection is a promising alternative delivery method for nanoparticle based gene therapies as it provides greater access to the central nervous system and does not require the drug to cross the blood-brain barrier, a previously identified barrier to transport. In intrathecal administration, therapeutic is injected into the cerebrospinal fluid (CSF) contained within the subarachnoid space. Once injected, however, dynamics between the CSF itself and the therapeutic may limit transport, necessitating a deeper understanding of the CSF composition. While human CSF has been extensively studied, there is a lack of information regarding the composition of commonly used animal models. This research aims to develop a protocol for the cross-species characterization of electrolytes found in CSF (Na, Cl, K, Ca, Mg), to enhance our understanding of the environment in which nanoparticles are injected. The protocol incorporated test strips to determine initial dilution factors and estimate the concentrations. Colorimetric assays for chloride and potassium were performed for precise analysis of those electrolytes in CSF in a Yucatan minipig model. Preliminary results assess the variability between individuals of the same species. Additionally, the effects of centrifugation during the sample preparation were investigated. This research provides a characterization of electrolyte levels in the CSF of the Yucatan minipig model. Furthermore, it establishes a protocol that can be utilized for future studies involving other animal models and cross-species analysis of CSF components. Keywords: [no keywords provided]

Source:

Purdue University / 2024

Topics:

No topics listed

Co-authors:

Matthew William Margraf